Metabolomic profile of follicular fluid and embryo culture media in patients with extragenital endometriosis


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Abstract

Objective: To identify specif ic markers in follicular fluid (FF) and embryo culture media to assess embryo quality and implantation potential in patients with extragenital endometriosis (EGE). Materials and methods: We performed comparative genomic hybridization to analyze FF and embryo culture media samples from patients with EGE and control subjects undergoing the IVF (ICSI) with preimplantation genetic testing. Spent culture medium metabolites from individually cultured embryos were detected using the HPLC-MS in the positive ion detection mode. After identifying chromatographic peaks and aligning chromatograms, partial least squares discriminant analysis was performed to better visualize the metabolite prof iles of the compared samples. Results: Metabolite profiling revealed differences in FF and culture medium compositions of patients with EGE and control subjects. Comparative analysis of metabolites showed changes in lipid metabolism and decreased antioxidant levels in patients with EGE. These findings suggest an essential role of oxidative stress and lipid metabolism regulation, especially fatty acids performing structural, nutritional, and signaling functions in EGE pathogenesis. Conclusion: Further analysis of the study findings and the search for markers attributable to embryonic development features, characteristic of EGE, remain relevant.

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About the authors

Luiza K. Ibragimova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: ibragimova_luisa0693@mail.ru
postgraduate student, Professor BV. Leonov Department of IVF

Veronika Yu. Smol'nikova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Dr. Med. Sci., Leading Researcher at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Chupalav M. El'darov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

PhD. (Bio), Senior Researcher at the Laboratory of Molecular Pathophysiology

Mikhail Yu. Bobrov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: mbobr@mail.ru
PhD. (Chemistry), Head of the Laboratory of Molecular Pathophysiology

Diana S. Agadzhanyan

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: agadzhanyan@inbox.ru
Post-graduate Student at the B.V. Leonov Department of Assisted Technologies for the Treatment of Infertility

Evgeny A. Romanov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Embryologist at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Elena A. Kalinina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
Dr. Med. Sci., Professor, Head of the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

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