Assessment of the expression level of hsa_pir_020497 piRNA in the follicular fluid of patients with different in vitro fertilization outcomes


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Abstract

Objective: To determine the relationship between morphological and molecular biological criteria for assessing the quality of oocytes retrieved from the women on the day of transvaginal puncture of follicles. Materials and methods: We used deep sequencing method with its subsequent validation by real-time RT-PCR to identify the spectrum of small non-coding RNAs (sncRNAs) present in the follicular fluid of the left and right ovaries of 25 patients; among them, three patients underwent IVF program again after a negative result of selective embryo transfer into the uterine cavity and preconception care (administration of myo-inositol in combination with folic acid, vitamin D and omega-3). Results: We identified 86 piRNAs and 79 microRNAs by deep sequencing of sncRNA of the ovarian follicular fluid; expression level of hsa_pir_020497piRNA was statistically significantly (p=0.01) higher in the group of patients with a positive ART treatment outcome (n=14) compared to the group of patients with a negative ART treatment outcome (n=8) according to real-time RT-PCR data. After preconception care, all three patients showed an increase in the expression level of hsa_piR_020497in the follicular fluid in one or two ovaries to the expression level of hsa_piR 020497 which is characteristic of pregnant women. Protein products of potential target genes hsa_piR_020497participate in signaling pathways mediated by the epidermal growth factor receptor (ErbB), mitogen-activated protein kinase (MAPK), transforming growth factor beta (TGFP), insulin-like growth factor 1 (IGF-1), phosphatidylinositol-3-kinase/Akt pathway and in the regulation of cytosolic calcium and metabolism of vitam ins and cofactors. Conclusion: Since the protein products of the target genes hsa_piR_020497 are involved in the main signaling pathways responsible for follicle formation and egg maturation, it is relevant to conduct further studies on a large sample of couples to determine its impact on IVF outcomes.

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About the authors

Maria A. Shamina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: mariashamina@mail.ru
PhD student at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Angelika V. Timofeeva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: avtimofeeva28@gmail.com
MD., Ph.D., Head of Laboratory of applied transcriptomics

Ivan S. Fedorov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: is_fedorov@oparina4.ru
Junior Researcher at the Laboratory of Applied Transcriptomics at the Department of Systems Biology

Elena A. Kalinina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
Dr. Med. Sci., Professor, Head of the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

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