Impact of preimplantation genetic testing on assisted reproductive technology outcomes in couples with male factor infertility


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Abstract

Background: In recent years, considerable attention has been focused on the association between severe male factor (SMF) and the incidence of embryonic aneuploidy, including whether SMF should be considered an indication for preimplantation genetic testing for aneuploidy (PGT-A). Objective: To investigate the impact of men's age and form of male infertility on the rate of embryonic aneuploidy and the outcomes of assisted reproductive technology (ART). Materials and methods: This retrospective study analyzed 2915 ART cycles (2225 stimulation cycles, including 371 cycles with PGT-A and 690 cryopreserved cycles). The ejaculate was evaluated based on the sperm quality criteria of the WHO reference values. The SMF group consisted of patients with oligoasthenoteratozoospermia and patients with testicular biopsy. Patients with teratozoospermia were divided into two groups, categorized by the percentage of morphologically abnormal spermatozoa. On day five after fertilization, the embryo trophectoderm was biopsied, followed by PGT-A. Results: Comparison of ART outcomes in stimulation cycles and a fresh embryo transfer and in cryopreserved cycles with and without PGT-A showed statistically significantly lower pregnancy and birth rates in patients with the sperm morphology score of 0-2% and 3% in cryopreserved cycles without PGT-A. Patients with SMF undergoing ART with PGT-A showed a trend towards increasing pregnancy and birth rates. Conclusion: PGT-A can improve pregnancy outcomes for couples with SMF with fewer embryos transferred due to reducing early pregnancy losses.

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About the authors

Natalia P. Makarova

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: np_makarova@oparina4.ru
Dr. Biol. Sci., Senior Researcher at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Nataliya N. Lobanova

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Junior Researcher at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Elena V. Kulakova

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kulakova@oparina4.ru
PhD, Senior Researcher, Professor BV. Leonov Department of IVF

Oksana S. Nepsha

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: o_nepsha@oparina4.ru
PhD. (Biol. Sci.), Researcher at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Alexey N. Ekimov

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_ekimov@oparina4.ru
doctor-laboratory geneticist, Head of the Group of preimplantation genetic screening of the Laboratory of Molecular Genetic Methods

Elena A. Kalinina

V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
Dr. Med. Sci., Professor, Head of the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

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