Improvement of the embryological stage in assisted reproduction treatment with hyaluronan-enriched culture medium in couples using preimplantation genetic testing for aneuploidy
- Authors: Drapkina Y.S.1, Kulakova E.V.1, Nepsha O.S.1, Ekimov A.N.1, Makarova N.P.1, Kalinina E.A.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Issue: No 11 (2021)
- Pages: 166-174
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/249435
- DOI: https://doi.org/10.18565/aig.2021.11.166-174
- ID: 249435
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Abstract
Background: During the transfer of an euploid embryo, implantation failure can result from the inadequate formation of the embryo-endometrial adhesion. According to the studies, hyaluronic acid culture media have a positive effect on the outcomes of assisted reproduction programs due to a better embryo-endometrial communication. Such media can also influence the processes of endometrial decidualization and embryo development. Objective: To evaluate pregnancy and delivery rates in patients undergoing infertility treatment with IVF/ ICSI with preimplantation genetic testing for aneuploidy (PGT-A) when an euploid embryo is transferred in hyaluronan-enriched culture medium. Materials and methods: This was a retrospective analysis of the data of309 couples undergoing IVF/ICSI cycles with gonadotropin-releasing hormone (GnRH) antagonist protocol. Cyclic hormone therapy was administered to prepare the endometrium for the subsequent transfer of an euploid embryo. Hyaluronan-enriched culture medium was used in embryo transfer of 53 patients and a standard culture medium was used in 256 cases. Results: Depending on the age, all patients were divided into three subgroups: subgroup 1 (24-30 years, 72 women), subgroup 2 (31-36 years, 148 women), subgroup 3 (37-43 years, 89 women). Unlike the results of other studies, we did not find any statistically significant effect of the hyaluronan-enriched culture medium on the outcomes of ART programs in patients of different age subgroups. Conclusion: The absence of statistically significant results may be associated with a small sample of patients in the group with hyaluronic acid, as well as the criteria for selecting patients in the comparison and control group. Further research should be done to investigate the optimal use of hyaluronan-enriched culture medium and to determine the indications for its use in different groups of patients.
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About the authors
Yulia S. Drapkina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: yu_drapkina@oparina4.ru
PhD, researcher, Professor BV. Leonov Department of IVF
Elena V. Kulakova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: e_kulakova@oparina4.ru
PhD, Senior Researcher, Professor BV. Leonov Department of IVF
Oksana S. Nepsha
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: o_nepsha@oparina4.ru
PhD. (Biol. Sci.), Researcher at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility
Alexey N. Ekimov
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: a_ekimov@oparina4.ru
doctor-laboratory geneticist, Head of the Group of preimplantation genetic screening of the Laboratory of Molecular Genetic Methods
Natalia P. Makarova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: np_makarova@oparina4.ru
Dr. Biol. Sci., Senior Researcher at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility
Elena A. Kalinina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: e_kalinina@oparina4.ru
Dr. Med. Sci., Professor, Head of the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility
References
- Vander B.M., Wyns C. Fertility and infertility: Definition and epidemiology. Clin. Biochem. 2018; 62: 2-10. https://dx.doi.org/10.1016/j.clinbiochem.2018.03.012.
- Singh N., Gupta M., Kriplani A., Vanamail P. Role of embryo glue as a transfer medium in the outcome of fresh non-donor in-vitro fertilization cycles. J. Hum. Reprod. Sci. 2015; 8(4): 214-7. https://dx.doi.org/10.4103/0974-1208.170398.
- Behzad F., Seif M. W., Campbell S., Aplin J.D. Expression of 2 isoforms of CD44 in human emdometrium. Biol. Reprod. 1994; 51(4): 739-47. https://dx.doi.org/10.1095/biolreprod51.4.739.
- Berneau S.C., Ruane P.T., Brison D.R., Kimber S.J., Westwood M., Aplin J.D. Investigating the role of CD44 and hyaluronate in embryo-epithelial interaction using an in vitro model. Mol. Hum. Reprod. 2019; 25(5): 265-73. https://dx.doi.org/10.1093/molehr/gaz011.
- Wu F., Lu R., Bai X.H., Song X.R. Influence of EmbryoGlue on the implantation of embryo and pregnancy outcome in vitro fertilization-embryo transfer. Zhonghua Fu Chan Ke Za Zhi. 2012; 47(2): 121-4. (in Chinese).
- Girish K.S., Kemparaju K. The magic glue hyaluronan and its eraser hyaluronidase: a biological overview. Life Sci. 2007; 80(21): 1921-43. https://dx.doi.org/10.1016/j.lfs.2007.02.037.
- Lim K., Lee B., Kang S., Hwang W. Effects of protein source and energy substrates on the in vitro development of bovine embryos in a two-step culture system. J. Vet. Sci. 2003; 4(1): 73-8.
- Valojerdi M.R., Karimian L., Yazdi P.E., Gilani M.A., Madani T., Baghestani A.R. Efficacy of a human embryo transfer medium: a prospective, randomized clinical trial study. J. Assist. Reprod. Genet. 2006; 23(5): 207-12. https://dx.doi.org/10.1007/s10815-006-9031-7.
- Bontekoe S., Heineman M., Johnson N., Blake D. Adherence compounds in embryo transfer media for assisted reproductive technologies. Cochrane Database Syst. Rev. 2014; (2): CD007421. https://dx.doi.org/10.1002/14651858.CD007421.pub3.
- Fu W., Yu M., Zhang X.J. Effect of hyaluronic acid-enriched transfer medium on frozen-thawed embryo transfer outcomes. J. Obstet. Gynaecol. Res. 2018; 44(4): 747-55. https://dx.doi.org/10.1111/jog.13581.
- Fancsovits P., Lehner A., Murber A., Kaszas Z., Rigo J., Urbancsek J. Effect of hyaluronan-enriched embryo transfer medium on IVF outcome: a prospective randomized clinical trial. Arch. Gynecol. Obstet. 2015; 291(5): 1173-9. https://dx.doi.org/10.1007/s00404-014-3541-9.
- Safari S., Razi M.H., Safari S., Razi Y. Routine use of EmbryoGlue as embryo transfer medium does not improve the ART outcomes. Arch. Gynecol. Obstet. 2015; 291(2): 433-7. https://dx.doi.org/10.1007/s00404-014-3416-0.
- Ruane P.T., Buck C.J., Babbington P.A., Aboussahoud W., Berneau S.C., Westwood M. et al. The effects of hyaluronate-containing medium on human embryo attachment to endometrial epithelial cells in vitro. Hum. Reprod. Open. 2020; 2020(2): hoz033. https://dx.doi.org/10.1093/hropen/hoz033.
- Драпкина Ю.С., Тимофеева А.В., Чаговец В.В., Кононихин А. С., Франкевич В.Е., Калинина Е.А. Применение омиксных технологий в решении проблем репродуктивной медицины. Акушерство и гинекология. 2018; 9: 24-32. [Drapkina Yu.S., Timofeeva A.V., Chagovets V.V., Kononikhin A.S., Frankevich V.E., Kalinina E.A. Application of omix technologies in solving problems of reproductive medicine. Obstetrics and gynecology. 2018; 9: 24-32. (in Russian)]. https://dx.doi.org/10.18565/aig.2018.9.24-32.
- Приказ Минздрава России от 31.07.2020 № 803н "О порядке использования вспомогательных репродуктивных технологий, противопоказаниях и ограничениях к их применению". Настоящий приказ вступает в силу с 1 января 2021 года.
- Андреева М.Г., Калинина Е.А., Дьяконов С.А. Критерии успеха в программах вспомогательных репродуктивных технологий. Акушерство и гинекология. 2016; 3: 12-5. https://dx.doi.org/10.18565/aig.2016.3.12-15.
- Тимофеева А.В., Калинина Е.А., Драпкина Ю.С., Чаговец В.В., Макарова Н.П., Сухих Г.Т. Оценка качества эмбриона по профилю экспрессии малых некодирующих РНК в культуральной среде эмбриона в программах вспомогательных репродуктивных технологий. Акушерство и гинекология. 2019; 6: 79-86. https://dx.doi.org/10.18565/aig.2019.6.79-86.
- Adeniyi T., Horne G., Ruane P.T., Brison D.R., Roberts S.A. Clinical efficacy of hyaluronate-containing embryo transfer medium in IVF/ICSI treatment cycles: a cohort study. Hum. Reprod. Open. 2021; 2021(1): hoab004. https://dx.doi.org/10.1093/hropen/hoab004.
- Fouladi-Nashta A.A., Raheem K.A., Marei W.F., Ghafari F., Hartshorne G.M. Regulation and roles of the hyaluronan system in mammalian reproduction. Reproduction. 2017; 153(2): R43-58. https://dx.doi.org/10.1530/REP-16-0240.
- Nakagawa K., Takahashi C., Nishi Y., Jyuen H., Sugiyama R., Kuribayashi Y. et al. Hyaluronan-enriched transfer medium improves outcome in patients with multiple embryo transfer failures. J. Assist. Reprod. Genet. 2012; 29(7): 679-85. https://dx.doi.org/10.1007/s10815-012-9758-2.
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