Lypidome analysis of cervicovaginal fluid (CVF) in patients with vulvovaginal atrophy under exposure to dynamic quadripolar radiofrequency
- Authors: Kazakova S.N.1, Apolokhina I.A.1,2, Chagovets V.V.1, Frankevich V.E.1,3, Teterina T.A.1, Tokareva A.O.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)
- Siberian State Medical University, Ministry of Health of Russia
- Issue: No 12 (2022)
- Pages: 132-138
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/250490
- DOI: https://doi.org/10.18565/aig.2022.251
- ID: 250490
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Abstract
Objective: To study specific features of cervicovaginal fluid lipidome in patients with vulvovaginal atrophy (VVA) in postmenopause under exposure to dynamic quadripolar radiofrequency (DQRF). Materials and methods: The study included 60patients aged 46- 65 years (the mean age 54.8 (5.1) years) with the symptoms of VVA. All patients underwent treatment: dynamic quadripolar radiofrequency was used in the area of vulva and vagina in group 1 (4 procedures with 2-week intervals); DQRF with radioporation technique with application of cream containing 0.5 mg estriol was used in group 2 (4 procedures with 2-week intervals); and local hormonal treatment with cream containing 0.5 mg estriol was used in group 3 (8 weeks of treatment). Lipid extraction from cervicovaginal fluid was performed using modified Folch method at two-time points - before and 1 month after treatment. Results: Lipidome analysis compared 3 groups of samples of cervicovaginal fluid- in 40 patients before and after exposure to DQRF and in 20 patients before and after treatment with local estrogens according to treatment regimen. 6 compounds were isolated. Their levels were statistically significantly higher in the group of patients who were exposed only to radiofrequency - Anandamide (18:2, n-6), DG 18:0/18:0/0:0, DG 18:0/16: 0/0:0, LTB4-dimethylamide, N-hydroxy arachidonoilamine, Virodhamin. The levels of Anandamide (18:2, n-6), LTB4-dimethylamide, N-hydroxy arachidonoilamine and Virodhamin were statistically significantly lower in patients who were exposed to radiofrequency in combination with estrogens for treatment of VVA in postmenopause. Conclusion: This article describes an innovative approach to alternative treatment of women with VVA in postmenopause using DQRF. The results of lipidome analysis of cervicovaginal fluid after exposture to DQRF are published for the first time. Further study and research in the field of high energy methods for VVA treatment are necessary to assess the effectiveness and safety of long term radio wave exposure.
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About the authors
Svetlana N. Kazakova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russiapostgraduate student at the Department of Obstetrics, Gynecology, Perinatology and Reproductology
Inna A. Apolokhina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)
Email: i_apolikhina@oparina4.ru
Dr. Med. Sci., Professor, Head of the Department of Aesthetic Gynecology and Rehabilitation
Vitaliy V. Chagovets
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of RussiaPhD, Head of the Laboratory of Metabolomics and Bioinformatics, Department of Systems Biology in Reproductive Medicine of the Institute of Translational Medicine
Vladimir E. Frankevich
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; Siberian State Medical University, Ministry of Health of Russia
Email: v_frankevich@oparina4.ru
Dr. Sci. (Physics and Mathematics), Deputy Director for Research - Head of the Department of Systems Biology in Reproduction, Institute of Translational Medicine
Tatiana A. Teterina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: t_teterina@oparina4.ru
PhD, Obstetrician-gynecologist, Department of Aesthetic Gynecology and Rehabilitation
Alisa O. Tokareva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: alisa.tokareva@phystech.edu
specialist at the Laboratory of Clinical Proteomics
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