Prevalence and importance of autoantibodies for non-invasive diagnostics of external genital endometriosis in women


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Abstract

External genital endometriosis (EGE) is a common estrogen-dependent chronic inflammatory disease in women of reproductive age, which is associated with endocrine disorders, immune dysregulation and formation of autoantibodies being considered as potential biomarkers of EGE Aim: To explore the profile and diagnostic significance of serum autoantibodies in women with EGE. Materials and methods: The antibodies against tropomyosin-3 (TPM-3), tropomodulin-3 (TMOD-3), α-enolase (a-ENO), estradiol (E), progesterone (PG), human chorionic gonadotropin (hCG), laminin-1, and antiphospholipid antibodies were measured using the enzyme-linked immunosorbent assay (ELISA) in 74patients with EGE stages III-IV and in 27 healthy women. Results: In patients with EGE, the antibodies (M, G) against E, PG, hCG, TPM, TMOD and ENO were found more often (28.4-50%) versus the comparison group (7.4-18,5%); the average antiobody levels (Mand/or G) in patients with EGE were higher. IgM against TPM, E, PG and hCG had a high diagnostic significance (AUC 0.721-0.847); diagnostic accuracy for combined tests was 80-84.8%. Conclusion: In patients with EGE high rates of detection of antibodies against E, PG, hCG, TPM, TMOD and ENO were noted, that may be pathogenetic factors of endometriosis. The antibodies against TPM and hormones have a high diagnostic significance and are prospective biomarkers for non-invasive diagnostics of endometriosis.

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About the authors

Irina V. Menzhinskaya

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation

Email: lmenzinskaya@oparina4.ru
Dr. Med. Sci., Leading Researcher, Laboratory of Clinical Immunology

Arika G. Melkumyan

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation

Email: dr.melkumyan@gmail.com
PhD student

Stanislav V. Pavlovich

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation

Email: s_pavbvich@oparina4.ru
PhD, Academic Secretary

Vladimir D. Chuprynin

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation

Email: v_chuprynin@oparina4.ru
PhD, Head of the Surgical Department

Lubov V. Krechetova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation

Email: l_krechetova@oparina4.ru
Dr. Med. Sci., Head of the Laboratory of Clinical Immunology

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