Uterine microbiome and immunohistochemical markers of chronic endometritis in recurrent pregnancy loss


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Abstract

Objective: To identify the genera of microorganisms that inhabit endometrium of healthy women and patients with recurrent pregnancy loss (RPL), as well as the genera of microorganisms causing chronic endometritis (CE). Materials and methods: The endometrial microbiome was examined in 14 women with RPL and 15 fertile healthy women by sequencing the next generation 16S rRNA gene. In addition, correlation between CE, CD138, CXCL13 markers and members of various endometrial bacterial genera was analyzed. Results: In patients of both groups, the most abundant bacterial genus in the endometrial samples was Lactobacillus (30.3% in patients with RPL, 29.3% in fertile patients). Statistically significant differences between the groups were found only for the bacterial genera Brevibacillus and Corynebacterium 1. The mean relative abundance of Brevibacillus was higher in the healthy fertile women [0.11 (0;0.3)%] than in the women with RPL [0 (0;0)% (p=0.008)]. The relative abundance of Corynebacterium 1 was 0 (0;0)% and 0.07 (0;0.13)% (p=0.002), respectively. The marker CD 138 was associated with the presence of the bacteria of genus Pseudorhodoferax. The presence of marker CXCL13 was associated with bacteria of genera Alistipes, Butyricimonas, Dialister, Leuconostoc, Neisseria, Parabacteroides, Phascolarctobacterium, Prevotella, Ruminococcaceae, Sutterella, Sphingobium, Subdoligranulum. Conclusion: Simultaneous analysis of the endometrial microbiome and CE markers can optimize preconception care in women with high perinatal risk.

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About the authors

Viktoriya V. Barinova

Rostov State Medical University; Clinic of Professor Bushtyreva LLC

Email: victoria-barinova@yandex.ru
PhD, Teaching Assistant at the Department of Obstetrics and Gynecology No. 1 Rostov-on-Don

Natalya B. Kuznetsova

Rostov State Medical University; Clinic of Professor Bushtyreva LLC

Email: lauranb@inbox.ru
Dr. Med. Sci., Professor at the Center for Simulation Training Rostov-on-Don

Irina O. Bushtyreva

Clinic of Professor Bushtyreva LLC

Email: kio4@mail.ru
Dr. Med. Sci., Professor, Director Rostov-on-Don

Vasilisa V. Dudurich

Medical Genetics Center Serbalab

Email: vdudurich@cerbalab.ru
Biologist-Geneticist, Director for Development

Alexander E. Shatalov

Kuban State Medical University

Email: shatal321@mail.ru
1st year resident at the Department of Obstetrics, Gynecology and Perinatology of the FPK and PPS

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