Mitochondrial DNA expression profile in embryo culture medium in assisted reproductive technology


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Abstract

Objective: To investigate the feasibility of using quantitative assessment of the MT-ND1 gene copy number in culture medium as a marker of successful implantation and embryo quality. Materials and methods: We analyzed the level of MT-ND1 gene copy number in the spent culture medium as a function of embryo quality, maternal age, and the outcome of embryo transfer into the uterine cavity. Results: A total of 142 embryos were obtained, including 102 blastocysts and 40 embryos with arrested development. There was no statistically significant difference between these groups in the distribution of mtDNA copies (p=0.919). Depending on morphological characteristics, all blastocysts were divided into Group 1 (60 excellent and good embryos) and Group 2 (42 average and poor embryos). The distribution of mtDNA copies in these groups did not differ statistically significantly (p=0.082). All blastocysts were divided into groups depending on patient age, including Group 1 (<35years, 63 embryos) and Group 2 (>35.39years, embryos). The number of mtDNA copies was statistically significantly higher in the group of patients aged under 35 (p=0.001). Embryo transfer was performed in 24patients. Depending on the embryo transfer outcome, patients were divided into Group 1 (negative result, 17 patients) and Group 2 (clinical pregnancy, seven patients). The distribution of mtDNA copies in these groups had no significant difference (p=0.234). Conclusion: Spent culture medium contains mtDNA derived from embryos that can be detected and analyzed by quantitative PCR. The level of mtDNA in the spent culture medium might serve as a promising marker for selecting the best embryos for transfer into the uterine cavity.

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About the authors

Nataliya P. Makarova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: np_makarova@oparina4.ru
Dr. Bio. Sci., Dr. Bio. Sci., Leading Researcher at at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Olga I. Lisitsyna

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Postgraduate Student

Oksana S. Nepsha

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: o_nepsha@oparina4.ru
Ph.D., Researcher at the B.V. Leonov Department of Assisted Technologies for the Treatment of Infertility

Aleksey M. Krasnyi

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_krasnyi@oparina4.ru
PhD. (Bio. Sci.), Head of the Cytology Laboratory

Alsu A. Sadekova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: sialsad@gmail.com
PhD (Bio), Researcher of the Cytology Laboratory

Alexandra L. Nezlina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: nezlina@vk.com
Junior Researcher at the Cytology Laboratory

Nataliya V. Dolgushina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Dr. Med. Sci., Professor, Deputy Director - Head of the Department of Research Administration

Boris V. Zingerenko

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: regniz@mail.ru
Researcher at the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Elena A. Kalinina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
Dr. Med. Sci., Professor, Head of the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

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