The content of different forms of E-cadherin in blood plasma and placental tissue in preeclampsia

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Objective: To determine the content of the adhesion molecule E-cadherin in the plasma and placenta of women with preeclampsia (PE) and assess its involvement in the development of PE.

Materials and methods: This study included 55 pregnant women. Thirty-five of them had pregnancies complicated by PE (Group I), and 20 had uncomplicated pregnancies (Group II, control). Blood plasma levels of the soluble form of E-cadherin were determined using enzyme immunoassay. Placental E-cadherin expression was studied by the Western blot method.

Results: The plasma soluble form of E-cadherin was higher in patients with PE than in controls, 8.0 ng/ml and 3.87 ng/ml, respectively (p<0.001). Placental tissue analysis showed a significantly higher content of the soluble form of E-cadherin in PE.

Conclusion: The increased level of the soluble form of E-cadherin in the plasma and placental tissue in PE reflects the abnormal proliferation and differentiation of trophoblasts, which is typical of this pathology.

Full Text

Restricted Access

About the authors

Artemiy P. Shelekhin

I.M. Sechenov First Moscow State Medical University of Ministry of Health of Russia (Sechenov University)

Author for correspondence.
Email: dr.shelekhin@gmail.com
ORCID iD: 0000-0002-7682-1329

Postgraduate Student

Russian Federation, Moscow

Oleg R. Baev

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation; I.M. Sechenov First Moscow State Medical University of Ministry of Health of Russia (Sechenov University)

Email: o_baev@oparina4.ru
ORCID iD: 0000-0001-8572-1971

Dr. Med. Sci., Professor, Head of the 1st Maternity Department, Academician V.I. Kulakov NMRС for OG&P, Ministry of Health of Russia

Russian Federation, Moscow; Moscow

Alsu A. Sadekova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation

Email: sialsad@gmail.com
ORCID iD: 0000-0003-4726-7477

PhD, Senior Researcher at the Cytology Laboratory

Russian Federation, Moscow

Diana N. Kokoeva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation

Email: Dikokoeva@mail.ru

Junior Researcher at the Cytology Laboratory

Russian Federation, Moscow

Alexey M. Krasnyi

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation

Email: a_krasnyi@oparina4.ru
ORCID iD: 0000-0001-7883-2702

PhD, Head of the Cytology Laboratory

Russian Federation, Moscow

References

  1. Karapetian А.О., Baev О.R., Sadekova А.А., Krasnyi А.М., Sukhikh G.T. Cell-free foetal DNA as a useful marker for preeclampsia prediction. Reprod. Sci. 2021; 28(5): 1563-9. https://dx.doi.org/10.1007/s43032-021-00466-w
  2. MacDonald T.M., Walker S.P., Hannan N.J., Tong S., Kaitu’u-Lino T.J. Clinical tools and biomarkers to predict preeclampsia. EBioMedicine. 2022; 75: 103780. https://dx.doi.org/10.1016/j.ebiom.2021.103780
  3. Miller D., Motomura K., Galaz J., Gershater M., Lee E.D., Romero R., Gomez-Lopez N. Cellular immune responses in the pathophysiology of preeclampsia. J. Leukoc. Biol. 2022; 111(1): 237-60. https://dx.doi.org/10.1002/JLB.5RU1120-787RR
  4. Ander S.E., Diamond M.S., Coyne C.B. Immune responses at the maternal-fetal interface. Sci. Immunol. 2019; 4(31): eaat6114. https://dx.doi.org/10.1126/SCIIMMUNOL.AAT6114
  5. Шелехин А.П., Баев О.Р., Красный А.М. Роль молекул клеточной адгезии в патогенезе преэклампсии. Акушерство и гинекология. 2021; 6: 22-8. [Shelekhin A.P., Baev O.R., Krasnyi A.M. The role of cell adhesion molecules in the pathogenesis of preeclampsia. Obstetrics and Gynecology. 2021; (6): 22-8 (in Russian)]. https://dx.doi.org/10.18565/aig.2021.6.22-28
  6. Zhou W., Santos L., Dimitriadis E. Characterization of the role for cadherin 6 in the regulation of human endometrial receptivity. Reprod. Biol. Endocrinol. 2020; 18(1): 66. https://dx.doi.org/10.1186/S12958-020-00624-W
  7. Qiuling J., Fei S., Qi L., Juan Z., Yunjian W., Huamei Y. et al. Downregulated ribosomal protein L39 inhibits trophoblast cell migration and invasion by targeting E-cadherin in the placenta of patients with preeclampsia. FASEB J. 2021; 35(4): e21322. https://dx.doi.org/10.1096/FJ.202002061R
  8. Fedorova L., Gatto-Weis C., Smaili S., Khurshid N., Shapiro J.I., Malhotra D., Horrigan T. Down-regulation of the transcription factor snail in the placentas of patients with preeclampsia and in a rat model of preeclampsia. Reprod. Biol. Endocrinol. 2012; 10: 15. https://dx.doi.org/10.1186/ 1477-7827-10-15
  9. Luo F., Yue J., Li L., Mei J., Liu X., Huang Y. Narrative review of the relationship between the maternal-fetal interface immune tolerance and the onset of preeclampsia. Ann. Transl. Med. 2022; 10(12): 713. https://dx.doi.org/10.21037/ATM-22-2287
  10. Blechschmidt K., Mylonas I., Mayr D., Schiessl B., Schulze S., Becker K.F., Jeschke U. Expression of E-cadherin and its repressor snail in placental tissue of normal, preeclamptic and HELLP pregnancies. Virchows Arch. 2007; 450(2): 195-202. https://dx.doi.org/10.1007/S00428-006-0343-X
  11. Pęksa M., Kamieniecki A., Gabrych A., Lew‐tusk A., Preis K., Świątkowska‐freund M. Loss of E-cadherin staining continuity in the trophoblastic basal membrane correlates with increased resistance in uterine arteries and proteinuria in patients with pregnancy-induced hypertension. J. Clin. Med. 2022; 11: 668. https://dx.doi.org/10.3390/JCM11030668
  12. Li H.W., Cheung A.N.Y., Tsao S.W., Cheung A.L.M., O W.S. Expression of e-cadherin and beta-catenin in trophoblastic tissue in normal and pathological pregnancies. Int. J. Gynecol. Pathol. 2003; 22(1): 63-70. https://dx.doi.org/10.1097/00004347-200301000-00013
  13. Li X.L., Dong X., Xue Y., Li C.F., Gou W.L., Chen Q. Increased expression levels of E-cadherin, cytokeratin 18 and 19 observed in preeclampsia were not correlated with disease severity. Placenta. 2014; 35(8): 625-31. https://dx.doi.org/10.1016/j.placenta.2014.04.010
  14. Du L., Kuang L., He F., Tang W., Sun W., Chen D. Mesenchymal-to-epithelial transition in the placental tissues of patients with preeclampsia. Hypertens. Res. 2017; 40(1): 67-72. https://dx.doi.org/10.1038/hr.2016.97

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1

Download (56KB)
Indexing metadata
3. Fig. 2

Download (125KB)
Indexing metadata
4. Fig. 3

Download (69KB)
Indexing metadata

Copyright (c) 2023 Bionika Media

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies