Clinical characteristics and analysis of changes in amino acid and organic acid profiles in the urine of patients at risk of preeclampsia
- Authors: Оshkhunova M.S.1, Chagovets V.V.1, Novoselova A.V.1, Muminova K.T.1, Gorina K.A.1, Khodzhaeva Z.S.1, Frankevich V.E.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
- Issue: No 8 (2023)
- Pages: 56-66
- Section: Original Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/585243
- DOI: https://doi.org/10.18565/aig.2023.48
- ID: 585243
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Abstract
The etiology of preeclampsia remains unclear; however, pathophysiological changes in multiple metabolic pathways are observed in this condition. Studying urine metabolites in preeclampsia can help identify potential biomarkers for improved clinical diagnosis and enhance our understanding of the mechanisms underlying its development.
Objective: To determine the characteristic features of urine composition in pregnant women at risk of preeclampsia or in those who have developed preeclampsia.
Materials and methods: The study utilized urine samples collected from pregnant women at 11–14, 22–25, and 32–35 weeks of gestation. Patients were categorized into three groups: Group I consisted of 13 women with physiological pregnancies, Group II comprised 13 patients at high risk of developing preeclampsia, and Group III included five pregnant women with diagnosed preeclampsia. Clinical, laboratory, and instrumental methods, including extended combined first-trimester screening, were employed. Additionally, the composition of amino acids and organic acids in the urine was analyzed using liquid chromatography with mass spectrometric detection.
Results: Preeclampsia diagnosis was based on the 2021 clinical guidelines of the Ministry of Health of Russia. Group III exhibited a significantly higher incidence of complicated obstetric and gynecological history, including antenatal fetal death (n=2, 40%) and a history of preeclampsia (n=3, 60%), than Group II, where only one patient (7.7%) had a history of preeclampsia. The patients in the control group did not have a complicated reproductive history (p<0.001). Chronic arterial hypertension was more prevalent in Group III (n=4, 80%). Significant differences were observed in the levels of proline, aspartate, and malic acid when comparing Group I to Group III, as well as proline, alpha-ketoglutarate, and 3-hydroxy-3-methylglutaric acid when comparing Group II to Group III
Conclusion: This study identified specific urinary metabolites whose levels were significantly different in patients with a healthy pregnancy, patients at risk of developing preeclampsia, and those diagnosed with preeclampsia at different gestational ages. Urine metabolite profiling is a promising method for predicting pre-eclampsia.
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About the authors
Madina S. Оshkhunova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: madina.oshkhunova@mail.ru
PhD Student at the High-risk Pregnancy Department
Russian Federation, MoscowVitaliy V. Chagovets
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: vvchagovets@gmail.com
PhD, Head of the Laboratory of Metabolomics and Bioinformatics
Russian Federation, MoscowAnastasia V. Novoselova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_novoselova@oparina4.ru
Researcher at the Laboratory of Metabolomics and Bioinformatics
Russian Federation, MoscowKamilla T. Muminova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: kamika91@mail.ru
PhD, Researcher at the High Risk Pregnancy Department
Russian Federation, MoscowKsenia A. Gorina
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: k_gorina@oparina4.ru
PhD, Researcher at the High Risk Pregnancy Department
Russian Federation, MoscowZulfia S. Khodzhaeva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: zkhodjaeva@mail.ru
Dr. Med. Sci., Professor, Deputy Director of Obstetrics Institute
Russian Federation, MoscowVladimir E. Frankevich
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: v_frankevich@oparina4.ru
Dr. Sci. (Physics and Mathematics), Head of the Department of Proteomics of Human Reproduction Department
Russian Federation, MoscowReferences
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