Quantitative and qualitative determination of human papillomavirus DNA in women with cervical lesions
- Authors: Bayramova G.R.1, Andreev A.O.1, Ilyasova N.A.1, Tregubova A.V.1, Badlaeva A.S.1, Trofimov D.Y.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Issue: No 1 (2024)
- Pages: 102-109
- Section: Original Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/628879
- DOI: https://doi.org/10.18565/aig.2023.260
- ID: 628879
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Abstract
Objective: This study aimed to investigate the relationship between quantified human papillomavirus (HPV) viral load and the severity of cervical lesions.
Materials and methods: Data from 819 HPV-positive women aged 18–81 years were analyzed. Patients were categorized into three groups based on histologically verified diagnoses. Group 1 comprised 186 patients with chronic cervicitis, Group 2 included 341 patients with low-grade squamous intraepithelial lesions (LSIL), and Group 3 included 292 patients with high-grade squamous intraepithelial lesions (HSIL). HPV DNA testing was conducted by real-time PCR to determine the number of genomic equivalents of the virus. The HPV Kvant-21 reagent kit, designed for the detection, typing, and quantification of human papillomavirus DNA with low carcinogenic risk (HPV types 6, 11, 44) and high carcinogenic risk (HPV types 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82), was used for analysis.
Results: The study found that detection of a high viral load was associated with a statistically significantly higher risk of HSIL than LSIL, regardless of HPV genotype (OR=5.26; 95% CI 3.19–8.64). A similar trend was observed with moderate viral loads (OR=2.32; 95% CI 1.78–3.03). Furthermore, LSIL was significantly more common than HSIL when a low viral load was detected (OR=3.09; 95% CI 1.91–4.99). Additionally, a statistically significant positive relationship between the viral load and the degree of cervical involvement was identified for HPV genotypes 16, 18, 31, 39, and 44.
Conclusion: This study highlights the significance of HPV load as an important diagnostic marker. This suggests that the value of HPV load is currently underrated owing to challenges in interpreting the results of quantitative HPV DNA determination and the complexity of conducting research in this domain.
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About the authors
Guldana R. Bayramova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Author for correspondence.
Email: bayramova@mail.ru
ORCID iD: 0000-0003-4826-661X
Dr. Med. Sci., Merited Doctor of the Russian Federation, Professor at the Department of Obstetrics and Gynecology of the Department of Professional Education, Clinical Care Supervisor at the Research and Outpatient Department
Russian Federation, 117997, Moscow, Oparina str., 4Alexander O. Andreev
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: sasha.grash2010@yandex.ru
ORCID iD: 0000-0002-9835-440X
Ph.D. Student, specialty “obstetrics and gynecology”
Russian Federation, 117997, Moscow, Oparina str., 4Natalya A. Ilyasova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: bayramova@mail.ru
ORCID iD: 0000-0003-0665-3515
Researcher at the Department of International Cooperation, Obstetrician-Gynecologist at the Research and Outpatient Department
Russian Federation, 117997, Moscow, Oparina str., 4Anna V. Tregubova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: a_tregubova@oparina4.ru
ORCID iD: 0000-0003-4601-1330
Junior Researcher at the 1st Pathological Department
Russian Federation, 117997, Moscow, Oparina str., 4Alina S. Badlaeva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: bayramova@mail.ru
ORCID iD: 0000-0001-5223-9767
Junior Researcher at the 1st Pathological Department
Russian Federation, 117997, Moscow, Oparina str., 4Dmitry Yu. Trofimov
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: d_trofimov@oparina4.ru
Corresponding Member of the RAS, Professor, Dr. Bio. Sci., Director of the Institute of Reproductive Genetics
Russian Federation, 117997, Moscow, Oparina str., 4References
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