Changes in the composition of intestinal microbiota and their association with cortisol, melatonin, tumor necrosis factor-α, and interleukin-17 levels in women with idiopathic recurrent miscarriage

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Abstract

Objective: This study aimed to evaluate changes in the taxonomic composition of the intestinal microbiota and examine their relationship with serum levels of cortisol, melatonin, tumor necrosis factor-α (TNF-α), and interleukin-17 (IL-17) in women with idiopathic recurrent miscarriage (IRM).

Materials and methods: This study included 55 women with IRM and 60 women with healthy pregnancies. The taxonomic composition of the gut microbiota and the serum concentrations of cortisol, melatonin, TNF-α, and IL-17 were analyzed.

Results: The study found that women with IRM exhibited changes in the taxonomic composition of intestinal microbiota, characterized by a significant decrease in α-diversity of the bacterial community (Chao1 index p=0.014) and a decrease in the abundance of Bifidobacterium (p<0.001), Lachnospira (p=0.032), Roseburia (p=0.003), and Coprococcus (p=0.012). Additionally, there was an increase in the abundance of Ruminococcus (p<0.001) and Klebsiella (p=0.002). Significant correlations were observed between cortisol levels and the presence of Lachnospira bacteria (r=-0.51; p=0.002) and between melatonin and the presence of Coprococcus bacteria (r=-0.49; p=0.012). Correlations were also identified between TNF-α and IL-17 concentrations and the Chao1 index (r=-0.51, p=0.002; r=-0.54, p=0.001, respectively), TNF-α and the abundance of Ruminococcus bacteria (r=0.51; p=0.002), and IL-17 and the abundance of Bifidobacterium (r=-0.52; p=0.001).

Conclusion: Potential modifications to the gut microbiota may have preventive and therapeutic implications in women with IRM.

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About the authors

Lesya N. Gumenyuk

V.I. Vernadsky Crimean Federal University

Author for correspondence.
Email: lesya_gymenyuk@mail.ru
ORCID iD: 0000-0003-2785-3882

Dr. Med. Sci., Professor at the Department of Psychiatry, Narcology, Psychotherapy with a course of general and medical psychology, S.I. Georgievsky Medical Institute

Russian Federation, Simferopol

Mikhail V. Gavrilov

GUTA Clinic LLC

Email: centr.gyn@yandex.ru
ORCID iD: 0000-0002-3957-2087

Head of Operational Gynecology

Russian Federation, Moscow

Galina A. Puchkina

V.I. Vernadsky Crimean Federal University

Email: puchkina.g.a@mail.ru
ORCID iD: 0000-0002-8882-8317

Associate Professor at the Department of Obstetrics, Gynecology and Perinatology No. 1, S.I. Georgievsky Medical Institute

Russian Federation, Simferopol

Feride S. Asanova

V.I. Vernadsky Crimean Federal University

Email: feride.asanova.01.01@mail.ru
ORCID iD: 0009-0003-3919-6485

Student at the S.I. Georgievsky Medical Institute

Russian Federation, Simferopol

Diana R. Medzhitova

V.I. Vernadsky Crimean Federal University

Email: medzhitova9032001@mail.ru
ORCID iD: 0009-0008-8642-3851

Student at the S.I. Georgievsky Medical Institute

Russian Federation, Simferopol

Maxim D. Bordyugov

V.I. Vernadsky Crimean Federal University

Email: maksjing4@mail.ru
ORCID iD: 0009-0002-4031-8649

Student at the S.I. Georgievsky Medical Institute

Russian Federation, Simferopol

Vladislav A. Tishchenko

V.I. Vernadsky Crimean Federal University

Email: lacebrra2016@gmail.com
ORCID iD: 0009-0003-3344-0766

Student at the S.I. Georgievsky Medical Institute

Russian Federation, Simferopol

Ksenia A. Rudeva

V.I. Vernadsky Crimean Federal University

Email: rudeva00@mail.ru
ORCID iD: 0009-0001-1857-1157

Student at the S.I. Georgievsky Medical Institute

Russian Federation, Simferopol

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Supplementary files

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2. Fig. 1. Phylogenetic composition of the intestinal microbiota in patients with IPV and healthy women

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3. Fig. 2. Comparative analysis of the generic composition of the intestinal microbiota in patients with IPV and healthy women.

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