Lipid profiling of follicular fluid and blood plasma as a method of predicting pregnancy in women undergoing assisted reproductive treatment

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Background: Modifications of infertility treatment protocols in assisted reproductive technology (ART) programs based on the clinical and laboratory history of couples still have limitations in predicting their effectiveness. The search for minimally invasive markers of gamete quality and markers of the effectiveness of ART treatment is a relevant area at this stage of development of reproductive medicine.

Objective: To create a model for predicting pregnancy on the basis of the lipid profile of blood plasma and follicular fluid in patients undergoing ART treatment by means of liquid chromatography and mass spectrometry.

Materials and methods: The present study analyzed the clinical and anamnestic characteristics, as well as the lipid profile, of couples undergoing infertility treatment with standard ovarian stimulation in the ART department. Follicular fluid and blood plasma sampling followed by cryopreservation was performed on the day of puncture. The lipid composition of biological fluids was studied using liquid chromatography with tandem mass spectrometry. The lipid profile of the samples underwent analysis to develop OPLS-DA models.

Results: The study revealed statistically significant differences in the composition of follicular fluid and blood plasma depending on the outcome of the treatment in patients who had assisted reproduction. ROC curve analysis of the developed models showed that the model based on plasma lipid composition was more effective than the model based on follicular fluid (AUC=0.97, AUC=0.91, respectively).

Conclusion: The data of the present study are consistent with the results of world studies and confirm the potential of using the lipid composition of follicular fluid and blood plasma as markers of the effectiveness of ART programs.

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作者简介

Yulia Fortygina

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

编辑信件的主要联系方式.
Email: yu_fortygina@oparina4.ru
ORCID iD: 0000-0002-1251-0505

PhD student

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Yulia Drapkina

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: yu_drapkina@oparina4.ru
ORCID iD: 0000-0002-0545-1607

PhD, Researcher at the Department of IVF named after Professor B.V. Leonov

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Natalya Makarova

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: np_makarova@oparina4.ru
ORCID iD: 0000-0003-1396-7272

PhD, Leading Researcher at the Department of IVF named after Professor B.V. Leonov

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Elena Kulakova

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kulakova@oparina4.ru
ORCID iD: 0000-0002-4433-4163

Dr. Med. Sci., Senior Researcher at the Department of IVF named after Professor B.V. Leonov

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Anastasiia Sysoeva

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: sysoeva.a.p@gmail.com
ORCID iD: 0000-0002-6502-4498

PhD, Embryologist at the Department of IVF named after Professor B.V. Leonov

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Marina Yushina

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: gmarinanikolaevna@gmail.com

Junior Researcher at the Laboratory of Metabolomics and Bioinformatics

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Chupalav Eldarov

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: chup4lav@yandex.ru
ORCID iD: 0000-0003-4027-6469

Senior Researcher at the Laboratory of Metabolomics and Bioinformatics

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Vitaly Chagovets

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: vvchagovets@gmail.com
ORCID iD: 0000-0002-5120-376X

PhD (in Physics and Mathematics), Head of the Laboratory of Metabolomics and Bioinformatics

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Vladimir Frankevich

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_frankevich@oparina4.ru
ORCID iD: 0000-0002-9780-4579

Dr. Sci. (in Physics and Mathematics), Head of the Department for Systems Biology in Human Reproduction

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Elena Kalinina

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
ORCID iD: 0000-0002-8922-2878

Dr. Med. Sci., Professor, Head of the Department of IVF named after Professor B.V. Leonov

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

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2. Fig. 1. Score chart based on the results of OPLS-DA analysis of follicular fluid lipid levels. Red dots correspond to samples from patients who did not become pregnant, green dots to samples from patients who became pregnant.

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3. Fig. 2. ROC curve of the OPLS-DA model based on follicular fluid lipid levels

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4. Fig. 3. Score chart based on the results of OPLS-DA analysis of blood plasma lipid levels. Red dots correspond to samples from patients who did not become pregnant, green dots to samples from patients who became pregnant.

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5. Fig. 4. ROC curve of the OPLS-DA model based on plasma lipid levels

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