Criteria for assessing fetal neurogenesis dysfunction in early-onset growth restriction using extracellular vesicles
- Authors: Kan N.E.1, Leonova A.A.1, Gusar V.A.1, Chagovets V.V.1, Tyutyunnik V.L.1, Volochaeva M.V.1, Soldatova E.E.1, Ryzhova K.O.1, Serebriakova A.P.1
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Affiliations:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
- Issue: No 3 (2025)
- Pages: 56-64
- Section: Original Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/679753
- DOI: https://doi.org/10.18565/aig.2025.30
- ID: 679753
Cite item
Open Access
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Abstract
Objective: To evaluate neurogenesis dysfunction during early-onset fetal growth restriction by obtaining fetal neuronal exosomes (FNE) from maternal plasma, assessing the expression of neurotrophin proteins involved in neurogenesis and the regulation of fetal nervous system plasticity, and establishing relationships with clinical and functional data.
Materials and methods: This study included 45 pregnant women. The study group consisted of 20 women with early-onset fetal growth restriction, whereas the control group comprised 25 women with normal pregnancies. The gestational age of the newborns in both groups did not exceed 34 weeks. Extracellular vesicles were isolated from maternal plasma using a commercial kit, followed by immunoprecipitation to obtain FNE. The expression of neurotrophin proteins—nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF)—was assessed using Western blotting.
Results: A decrease in the expression of BDNF (pro-BDNF) and NGF (pro-NGF) precursors was observed in the FNE of the study group. Mature forms of neurotrophin proteins were not detected in FNE. Changes in pro-BDNF levels were noted only in cases of intraventricular hemorrhage (IVH), while pro-NGF levels varied in IVH, cerebral ischemia, and asphyxia in newborns during the neonatal period.
Conclusion: The results demonstrate, for the first time, the potential to detect brain neurodysfunction in fetuses with growth restriction by assessing the expression of neuronal proteins in FNE isolated from maternal blood via immunoprecipitation. Changes in these protein levels may reflect the degree of brain dysfunction and serve as potential prognostic and diagnostic markers of pathological conditions.
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About the authors
Natalia E. Kan
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: kan-med@mail.ru
ORCID iD: 0000-0001-5087-5946
SPIN-code: 5378-8437
ResearcherId: B-2370-2015
Professor, Dr. Med. Sci., Merited Scholar of the Russian Federation, Deputy Director for Research – Director of the Institute of Obstetrics
Russian Federation, 117997, Moscow, Ac. Oparin str., 4Anastasia A. Leonova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Author for correspondence.
Email: nastena27-03@mail.ru
ORCID iD: 0000-0001-6707-3464
PhD student
Russian Federation, 117997, Moscow, Ac. Oparin str., 4Vladislava A. Gusar
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: v_gusar@oparina4.ru
ORCID iD: 0000-0003-3990-6224
PhD, Senior Researcher at the Laboratory of Transcriptomic, Department of Systems Biology in Reproduction
Russian Federation, 117997, Moscow, Ac. Oparin str., 4Vitaliy V. Chagovets
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: vvchagovets@gmail.com
ORCID iD: 0000-0002-5120-376X
PhD, Senior Researcher at the Laboratory of Transcriptomic, Department of Systems Biology in Reproduction
Russian Federation, 117997, Moscow, Ac. Oparin str., 4Victor L. Tyutyunnik
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: tioutiounnik@mail.ru
ORCID iD: 0000-0002-5830-5099
SPIN-code: 1963-1359
Scopus Author ID: 56190621500
ResearcherId: B-2364-2015
Professor, Dr. Med. Sci., Leading Researcher at the Center for Scientific and Clinical Research
Russian Federation, 117997, Moscow, Ac. Oparin str., 4Maria V. Volochaeva
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: m_volochaeva@oparina4.ru
ORCID iD: 0000-0001-8953-7952
PhD, Senior Researcher at the Department of Regional Cooperation and Integration, Physician at 1 Maternity Department
Russian Federation, 117997, Moscow, Ac. Oparin str., 4Ekaterina E. Soldatova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: katerina.soldatova95@bk.ru
ORCID iD: 0000-0001-6463-3403
Researcher at the Obstetric Department of the Institute of Obstetrics
Russian Federation, 117997, Moscow, Ac. Oparin str., 4Kristina O. Ryzhova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: cr.yanina@gmail.com
ORCID iD: 0009-0007-8318-435X
Resident
Russian Federation, 117997, Moscow, Ac. Oparin str., 4Anna P. Serebriakova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia
Email: serebriakovanna@gmail.com
ORCID iD: 0000-0001-7014-2627
obstetrician-gynecologist of the Day Hospital Department
Russian Federation, 117997, Moscow, Ac. Oparin str., 4References
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