Characteristic effect of prednisolone in vitro administered in the form of prednisolone sodium phosphate in a combined preparation on biofilm-forming microorganisms of the vaginal biotope

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Abstract

Objective: To evaluate the effect of prednisolone in vitro administered in the form of prednisolone sodium phosphate in the Elzhina combined preparation on biofilm-forming microorganisms of the vaginal biotope.

Materials and methods: The study included clinical isolates forming the films of the following bacteria: Klebsiella pneumoniae (3), Escherichia coli (2), Enterococcus faecalis (3), Streptococcus agalactiae (1), Gardnerella vaginalis (8), Staphylococcus epidermidis (1), Candida albicans (5), Candida dubliniensis (1), Candida glabrata (1), Candida parapsilosis (1). There were 26 isolates. Dense and liquid selective nutrient media were used for cultivation, storage and further research. Microorganisms were identified using MALDI-TOF mass spectrometry (Bruker Microflex). The ability to form biofilms was assessed using a modified protocol of Christensen et al. (1985). The Elzhina preparation (solution of 1 tablet in 10 ml of physiological solution) and solutions of the main active ingredients of the preparation, i.e. neomycin, ornidazole, econazole, prednisolone (in the form of sodium phosphate), in concentrations corresponding to their content in the preparation were used to assess the effect on bacterial films.

Results: The Elzhina preparation showed a destructive effect on most biofilms that were formed by isolates of microorganisms included in the study (the coefficient of change of optical density after exposure to the Elzhina preparation was more than 1.0 in 96% of the isolates studied). The inclusion of prednisolone in the composition of the drug enhances the effect of the drug components in destroying biofilms formed by bacteria and yeast-like fungi. When determining the sensitivity of one isolate of K. pneumoniae to neomycin sulphate and four isolates of C. albicans to econazole in the presence of prednisolone, there was an increase in the zone of growth retardation around the inoculum with antibiotic towards the site of application of prednisolone sodium phosphate.

Conclusion: Prednisolone sodium phosphate, which has no direct antimicrobial and antifungal effect, has been shown to increase the antibacterial and antifungal effect of neomycin, ornidazole, econazole, contained in the Elzhina vaginal tablets, on bacteria and yeast-like fungi, as well as to increase the destructive effect of the preparation on bacterial films.

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About the authors

Tatiana A. Khusnutdinova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Author for correspondence.
Email: husnutdinovat@yandex.ru

PhD, Senior Researcher of the Experimental Microbiology Group, Teaching Assistant at the Department of Clinical Laboratory Diagnostics

Russian Federation, 199034, St. Petersburg, Mendeleevskaya Line, 3; 194100, St. Petersburg, Litovskaya str., 2

Elena V. Spasibova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: elena.graciosae@gmail.com
ORCID iD: 0009-0002-6070-4651

bacteriologist at the Laboratory of Clinical Microbiology, Teaching Assistant at the Department of Clinical Laboratory Diagnostics

Russian Federation, 199034, St. Petersburg, Mendeleevskaya Line, 3; 194100, St. Petersburg, Litovskaya str., 2

Kira V. Shalepo

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: 2474151@mail.ru
ORCID iD: 0000-0002-3002-3874

PhD, Senior Researcher at the Experimental Microbiology Group, Associate Professor at the Department of Clinical Laboratory Diagnostics

Russian Federation, 199034, St. Petersburg, Mendeleevskaya Line, 3; 194100, St. Petersburg, Litovskaya str., 2

Olga V. Budilovskaya

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: o.budilovskaya@gmail.com
ORCID iD: 0000-0001-7673-6274

PhD, Senior Researcher at the Experimental Microbiology Group, Teaching Assistant of the Department of Clinical Laboratory Diagnostics

Russian Federation, 199034, St. Petersburg, Mendeleevskaya Line, 3; 194100, St. Petersburg, Litovskaya str., 2

Anna A. Krysanova

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: krusanova.anna@mail.ru
ORCID iD: 0000-0003-4798-1881

PhD, Senior Researcher at the Experimental Microbiology Group, Teaching Assistant at the Department of Clinical Laboratory Diagnostics

Russian Federation, 199034, St. Petersburg, Mendeleevskaya Line, 3; 194100, St. Petersburg, Litovskaya str., 2

Alexandrа S. Cheberya

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: alexa-vorobjeva.09@yandex.ru
ORCID iD: 0009-0008-1091-5753

laboratory assistant researcher at Microbiology Laboratory

Russian Federation, 199034, St. Petersburg, Mendeleevskaya Line, 3

Alexander R. Cheberya

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology

Email: sanekcheberya@yandex.ru
ORCID iD: 0009-0006-9058-6720

laboratory assistant researcher at Microbiology Laboratory

Russian Federation, 199034, St. Petersburg, Mendeleevskaya Line, 3

Alevtina M. Savicheva

D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: savitcheva@mail.ru
ORCID iD: 0000-0003-3870-5930

Dr. Med. Sci., Professor, Head of the Department of Medical Microbiology, Head of the Department of Clinical Laboratory Diagnostics

Russian Federation, 199034, St. Petersburg, Mendeleevskaya Line, 3; 194100, St. Petersburg, Litovskaya str., 2

References

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2. Fig. 1. Effect of the drug "Elzhina" on bacterial films in vitro. Vertically - OP of the biofilm before and after the action of the drug "Elzhina", the curve reflects the coefficient of change in OP after the action of the drug "Elzhina"

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3. Fig. 2. The effect of ornidazole and its combination with prednisolone (in the form of prednisolone sodium phosphate) on bacterial films in vitro. The vertical axis shows the coefficient of change in OP after exposure.

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4. Fig. 3. The effect of econazole and its combination with prednisolone (in the form of prednisolone sodium phosphate) on bacterial films in vitro. The vertical axis shows the coefficient of change in OP after exposure.

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5. Fig. 4. The effect of neomycin and its combination with prednisolone (in the form of prednisolone sodium phosphate) on bacterial films in vitro. The vertical axis shows the coefficient of change in OP after exposure.

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6. Fig. 5. Clinical isolate of K. pneumoniae. Growth inhibition zones around the site of application of active substances (N - neomycin, E - econazole, O - ornidazole, P - prednisolone). On the left - the effect of neomycin sulfate in dilutions (from 1:1 to 1:1000). In the center - the effect of prednisolone (in the form of sodium phosphate) in dilutions (from 1:1 to 1:1000). On the right - the effect of all components of Elzhina (ornidazole, neomycin, econazole, prednisolone)

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