MICROBIOLOGICAL MONITORING OF PSEUDOMONAS AERUGINOSA AT OBSTETRIC HOSPITALS


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Abstract

Objective. To monitor Pseudomonas aeruginosa at obstetric hospitals, by taking into account the antibiotic resistance and genetic relationship of its strains. Subject and methods. The microorganisms were isolated from healthy neonatal infants (colonization) and in case of pyoseptic infections (PSI) in the period January 2006 to October 2012. The relationship of the isolated microorganisms was identif ied applying molecular genetic methods. Results. The mean annual infection rate over 5 years was 7.9±5.8 years per 1000 examinees. P. aeruginosae was most common in the meconium and gastric juice samples from newborn infants in case of colonization and in their expectorations in case of PSI. The infection most frequently occurs as exogenous, induced by nosocomial strains and, with due regard to the vulnerability of a contingent, shows an outbreak pattern. This assumption is evidenced by the antibiotic resistance of the isolated microorganisms and by blaOXA-50 gene sequencing. Conclusion. P. aeruginosa may be most important only in respiratory tract infection. The established uniformity of blaOXA-50 genes could suggest the formation of an epidemically significant clone in the closed circuit: an obstetric hospital — neonatal units of a children’s clinic.

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About the authors

M. V KUZNETSOVA

Institute for Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Acad. E.A. Vagner Perm State Medical Academy, Ministry of Health of the Russian Federation

Email: mar@iegm.ru

T. I KARPUNINA

Acad. E.A. Vagner Perm State Medical Academy, Ministry of Health of the Russian Federation

Email: karpuninapsma@mail.ru

E. S GOROVITS

Acad. E.A. Vagner Perm State Medical Academy, Ministry of Health of the Russian Federation

Email: Eduard.gorovitz@mail.ru

V. A DEMAKOV

Institute for Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences

Email: demakov@iegm.ru

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