STRUCTURAL FEATURES OF FETAL MEMBRANES IN PRETERM LABOR
- Authors: Nizyaeva N.V.1, Karapetian A.O.1, Gapaeva M.D.1, Sinitsyna V.A.1, Baev O.R.1,2
-
Affiliations:
- Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
- I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia
- Issue: No 8 (2019)
- Pages: 63-70
- Section: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248630
- DOI: https://doi.org/10.18565/aig.2019.8.63-69
- ID: 248630
Cite item
Open Access
Access granted
Abstract
Objective: to investigate structural alterations in the fetal membranes during preterm labor (PL) in the absence of inflammatory changes. Materials and methods. The fetal membranes of 25 placentas were analyzed: 14 from spontaneous PL at 28-36 weeks, 7 from term spontaneous labor during uncomplicated pregnancy; 4 from preterm operative delivery for fetal hypoxia, premature detachment of a normally situated placenta, and impaired fetal functional status at 27-32 weeks’ gestation. A histological (hematoxylin and eosin staining) and immunohistochemical studies on paraffin-embedded fetal membrane sections were performed using primary monoclonal antibodies to the mesenchymal cell marker vimentin (Spring Bioscience), to the marker of angiogenesis CD34 (Spring Bioscience), and to the macrophage marker CD68 (Spring Bioscience), as well as to the innate immunity receptor NOD-1 (Invitrogen). Immunohistochemical study was done using a closed Ventana immunostainer (Roche, UK). Results. Staining with antibodies to CD34, CD68, vimentin, and NOD-1 revealed the staining of mesenchymal cells and their processes, as well as macrophages located in the center of above structures. Increased angiogenesis in the compact layer and decidual plate was ascertained to weaken membrane mechanical strength during PB, and there were multiple defects and microfractures in these structures. Conclusion. Thus, the results of the investigation indicate that PL is due to a set of certain factors: decreased membrane mechanical strength, increased angiogenesis in the compact layer, and multiple microdefects and fractures, which lowers the mechanical strength of the fetal membranes.
Keywords
Full Text
About the authors
Natalia V. Nizyaeva
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: niziaeva@gmail.com
Ph.D., senior researcher, Pathology Department 117997, Russia, Moscow, Ac. Oparina str. 4
Anna O. Karapetian
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: a_karapetyan@oparina4.ru
physician of the first Maternity Department 117997, Russia, Moscow, Ac. Oparina str. 4
Masara D. Gapaeva
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: m_gapaeva@oparina4.ru
postgraduate student; first Maternity Department 117997, Russia, Moscow, Ac. Oparina str. 4
Veronica A. Sinitsyna
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: v_sinitsyna@oparina4.ru
senior laboratory assistant, Pathology Department 117997, Russia, Moscow, Ac. Oparina str. 4
Oleg R. Baev
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia
Email: o_baev@oparina4.ru
PhD, MD, Professor, Head of the first Maternity Department 117997, Russia, Moscow, Ac. Oparina str. 4
References
- Benirschke K., Burton G.J., Baergen R.N. Pathology of the human placenta. Sixth ed., Springer. 2012.
- Милованов А.П. Патология системы мать-плацента-плод: руководство для врачей. М.: Медицина; 1999. 448 с.
- Сухих Г.Т., Серов В.Н, Адамян Л.В. Преждевременные роды. Клинические рекомендации (протокол). М. 2014. 35o.
- Fahey J.O. Clinical management of intra-amniotic infection and chorioamnionitis: a review of the literature. J Midwifery Womens Health. 2008; 53(3): 227-235.
- Tita A.T., Andrews W.W. Diagnosis and management of clinical chorioamnionitis. Clin Perinatol. 2010; 37(2): 339-354.
- Steel J.H., Malatos S., Kennea N., Edwards A.D., Miles L., Duggan P., Reynolds P.R., Feldman R.G., Sullivan M.H. Bacteria and inflammatory cells in fetal membranes do not always cause preterm labor. Pediatr Res. 2005; 57(3): 404-411.
- Farraj A.A. Randomized placental and cord blood sampling culture in women with preterm and term labour to detect infection. East Mediterr Health J. 2000; 6: 272-275.
- Queiros da Mota V, Prodhom G., Yan P., Hohlfheld P., Greub G., Rouleau C. Correlation between placental bacterial culture results and histological chorioamnionitis: a prospective study on 376 placentas. J Clin Pathol. 2013; 66: 243-248.
- Низяева Н.В. Гистологические критерии воспалительных изменений плодных оболочек плаценты и пуповины. Международный журнал прикладных и фундаментальных исследований. 2018; 2: 202-207.
- Nizyaeva N., Karapetian A., Sinitsyna V., Pavlovich S., Kan N., Baev O. NOD-1 expression in placental villi in pregnancy-related complications. Virchows Archiv. 2018; 473: S1.
- Burton G.J., Sebire N.J., Myatt L., Tannetta D., Wang Y.L., Sadovsky Y. et al. Optimising sample collection for placental research. Placenta. 2014; 35: 9-22.
- Баев О.Р., Васильченко О.Н., Кан Н.Е., Клименченко Н.И., Митрохин С.Д., Тетруашвили Н.К., Ходжаева З.С., Шмаков Р.Г., Дегтярев Д.Н., Тютюнник В.Л., Адамян Л.В. Преждевременный разрыв плодных оболочек. Преждевременное излитие вод. Акушерство и гинекология. 2013; 9: 123-134.
- КанН.Е., СанниковаМ.В.,ДонниковА.Е., КлиманцевИ.В.,Амирасланов Э.Ю., Ломова Н.А., Кесова М.И., Костин П.А., Тютюнник В.Л., Сухих Г.Т. Клинические и молекулярно-генетические факторы риска преждевременного разрыва плодных оболочек. Акушерство и гинекология. 2013; 4: 14-18.
- Кан Н.Е., Санникова М.В., Амирасланов Э.Ю., Тютюнник В.Л. Клинические предикторы прогнозирования преждевременного разрыва плодных оболочек. Вопросы гинекологии, акушерства и перинатологии. 2013; 12(3):12-18.
- Kan N.E., Tuytyunnik V.L., Donnikov A.E., Sannikova M.V., Sukhikh G.T. Association of ESR1 gene polymorphism with preterm rupture of fetal membranes. Bulletin of Experimental Biology and Medicine. 2013; 156: 12: 811-814.
- Nizyaeva N.V., Sukhacheva T.V., Kulikova G.V., Nagovitsyna M.N., Poltavtseva R.A., Kan N.E., Tyutyunnik V.L., Pavlovich S.V., Serov R.A., Shchyogolev A.I. Ultrastructural Characteristics of Placental Telocytes. Bulletin of Experimental Biology and Medicine. 2017; 31.
- Lee S.M., Romero R., Park J.W., Kim S.M., Park C.W., Korzeniewski S.J., Chaiworapongsa T., Yoon B.H. The clinical significance of a positive Amnisure test in women with preterm labor and intact membranes. J Matern Fetal Neonatal Med. 2012; 25(9): 1690-98.
Supplementary files
