STRUCTURAL FEATURES OF FETAL MEMBRANES IN PRETERM LABOR
- Авторлар: Nizyaeva N.V.1, Karapetian A.O.1, Gapaeva M.D.1, Sinitsyna V.A.1, Baev O.R.1,2
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Мекемелер:
- Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
- I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia
- Шығарылым: № 8 (2019)
- Беттер: 63-70
- Бөлім: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248630
- DOI: https://doi.org/10.18565/aig.2019.8.63-69
- ID: 248630
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Аннотация
Objective: to investigate structural alterations in the fetal membranes during preterm labor (PL) in the absence of inflammatory changes. Materials and methods. The fetal membranes of 25 placentas were analyzed: 14 from spontaneous PL at 28-36 weeks, 7 from term spontaneous labor during uncomplicated pregnancy; 4 from preterm operative delivery for fetal hypoxia, premature detachment of a normally situated placenta, and impaired fetal functional status at 27-32 weeks’ gestation. A histological (hematoxylin and eosin staining) and immunohistochemical studies on paraffin-embedded fetal membrane sections were performed using primary monoclonal antibodies to the mesenchymal cell marker vimentin (Spring Bioscience), to the marker of angiogenesis CD34 (Spring Bioscience), and to the macrophage marker CD68 (Spring Bioscience), as well as to the innate immunity receptor NOD-1 (Invitrogen). Immunohistochemical study was done using a closed Ventana immunostainer (Roche, UK). Results. Staining with antibodies to CD34, CD68, vimentin, and NOD-1 revealed the staining of mesenchymal cells and their processes, as well as macrophages located in the center of above structures. Increased angiogenesis in the compact layer and decidual plate was ascertained to weaken membrane mechanical strength during PB, and there were multiple defects and microfractures in these structures. Conclusion. Thus, the results of the investigation indicate that PL is due to a set of certain factors: decreased membrane mechanical strength, increased angiogenesis in the compact layer, and multiple microdefects and fractures, which lowers the mechanical strength of the fetal membranes.
Негізгі сөздер
Толық мәтін
Авторлар туралы
Natalia Nizyaeva
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: niziaeva@gmail.com
Ph.D., senior researcher, Pathology Department 117997, Russia, Moscow, Ac. Oparina str. 4
Anna Karapetian
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: a_karapetyan@oparina4.ru
physician of the first Maternity Department 117997, Russia, Moscow, Ac. Oparina str. 4
Masara Gapaeva
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: m_gapaeva@oparina4.ru
postgraduate student; first Maternity Department 117997, Russia, Moscow, Ac. Oparina str. 4
Veronica Sinitsyna
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: v_sinitsyna@oparina4.ru
senior laboratory assistant, Pathology Department 117997, Russia, Moscow, Ac. Oparina str. 4
Oleg Baev
Academician V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia
Email: o_baev@oparina4.ru
PhD, MD, Professor, Head of the first Maternity Department 117997, Russia, Moscow, Ac. Oparina str. 4
Әдебиет тізімі
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