Evaluation of the in vitro effectiveness of the Depantol components on biofilms produced by vaginal microorganisms
- Authors: Shalepo K.V.1,2, Spasibova E.V.1,2, Budilovskaya O.V.1,2, Krysanova A.A.1,2, Khusnutdinova T.A.1,2, Cheberya A.S.1,3, Cheberya A.R.1,3, Savicheva A.M.1,2
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Affiliations:
- D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction
- Saint Petersburg State Pediatric Medical University, Ministry of Health of Russia
- S.M. Kirov Military Medical Academy
- Issue: No 10 (2024)
- Pages: 158-166
- Section: Exchange of Experience
- URL: https://journals.eco-vector.com/0300-9092/article/view/641939
- DOI: https://doi.org/10.18565/aig.2024.256
- ID: 641939
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Abstract
Objective: To evaluate the in vitro effectiveness of the Depantol components on biofilms produced by vaginal microorganisms.
Materials and methods: The study examined 33 clinical isolates of pure cultures of microorganisms obtained from the vaginal biotope. The bacterial films were produced by the following microorganisms: G. vaginalis (2 isolates), E. coli (3 isolates), K. pneumoniae (3 isolates), K. piersonii (1 isolate), C. freundii (3 isolates), A. baumannii (3 isolates), S. agalactiae (3 isolates), E. faecalis (3 isolates), S. aureus (3 isolates), and yeast-like fungi of Candida (9 isolates).
Dense and liquid selective nutrient media were used for the study. Microorganisms were identified using MALDI-TOF mass spectrometry (Bruker Microflex). The ability of microorganisms to produce biofilms was assessed according to a modified protocol of Christensen et al. (1985).
The in vitro effectiveness of the Depantol components on biofilms was evaluated using different dilutions of the drug components (chlorhexidine bigluconate, dexpanthenol, macrogols).
Results: The biofilms of varying density were formed by all 33 clinical isolates. Dexpanthenol in tested concentrations and macrogol had no effect on biofilms. Chlorhexidine bigluconate 1% solution destroyed biofilms produced by most vaginal bacteria. These are primarily G. vaginalis (both isolates tested), all E. coli, the clinical isolate of K. piersonii, all E. faecalis, S. aureus and all isolates of yeast-like fungi of Candida spp., both C. albicans and C. non-albicans (C. parapsilosis and C. glabrata). The biofilm was destroyed in two of the three isolates of K. pneumoniae, C. freundii, A. baumannii, and S. agalactiae.
Conclusion: Among the Depantol components, chlorhexidine bigluconate 1% solution was 100% effective on bacterial films produced by Gardnerella vaginalis, Escherichia coli, Klebsiella piersonii, Enterococcus faecalis, Staphylococcus aureus and yeast-like fungi of Candida spp., both C. albicans and C. non-albicans (C. parapsilosis and C. glabrata). Its effectiveness was 70% for the other microorganisms.
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About the authors
Kira V. Shalepo
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; Saint Petersburg State Pediatric Medical University, Ministry of Health of Russia
Author for correspondence.
Email: 2474151@mail.ru
ORCID iD: 0000-0002-3002-3874
PhD, Senior Researcher at the Department of Experimental Microbiology; Associate Professor at the Department of Clinical Laboratory Diagnostics
Russian Federation, Saint Petersburg; Saint PetersburgElena V. Spasibova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; Saint Petersburg State Pediatric Medical University, Ministry of Health of Russia
Email: elena.graciosae@gmail.com
ORCID iD: 0009-0002-6070-4651
Bacteriologist at the Laboratory of Clinical Microbiology; Teaching Assistant at the Department of Clinical Laboratory Diagnostics
Russian Federation, Saint Petersburg; Saint PetersburgOlga V. Budilovskaya
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; Saint Petersburg State Pediatric Medical University, Ministry of Health of Russia
Email: o.budilovskaya@gmail.com
ORCID iD: 0000-0001-7673-6274
PhD, Senior Researcher at the Department of Medical Microbiology; Teaching Assistant at the Department of Clinical Laboratory Diagnostics
Russian Federation, Saint Petersburg; Saint PetersburgAnna A. Krysanova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; Saint Petersburg State Pediatric Medical University, Ministry of Health of Russia
Email: krusanova.anna@mail.ru
ORCID iD: 0000-0003-4798-1881
PhD, Senior Researcher at the Department of Experimental Microbiology; Teaching Assistant at the Department of Clinical Laboratory Diagnostics
Russian Federation, Saint Petersburg; Saint PetersburgTatiana A. Khusnutdinova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; Saint Petersburg State Pediatric Medical University, Ministry of Health of Russia
Email: husnutdinovat@yandex.ru
ORCID iD: 0000-0002-2742-2655
PhD, Senior Researcher at the Department of Experimental Microbiology; Teaching Assistant at the Department of Clinical Laboratory Diagnostics
Russian Federation, Saint Petersburg; Saint PetersburgAlexandra S. Cheberya
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; S.M. Kirov Military Medical Academy
Email: alexa-vorobjeva.09@yandex.ru
ORCID iD: 0009-0008-1091-5753
Laboratory Assistant Researcher at Microbiology Laboratory; 6th-Year Student
Russian Federation, Saint Petersburg; Saint PetersburgAlexander R. Cheberya
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; S.M. Kirov Military Medical Academy
Email: sanekcheberya@yandex.ru
ORCID iD: 0009-0006-9058-6720
Laboratory Assistant Researcher at Microbiology Laboratory; 6th-Year Student
Russian Federation, Saint Petersburg; Saint PetersburgAlevtina M. Savicheva
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; Saint Petersburg State Pediatric Medical University, Ministry of Health of Russia
Email: savitcheva@mail.ru
ORCID iD: 0000-0003-3870-5930
Dr. Med. Sci., Professor, Head of the Department of Medical Microbiology; Head of the Department of Clinical Laboratory Diagnostics
Russian Federation, Saint Petersburg; Saint PetersburgReferences
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