Increased endometrial thickness and improved reproductive outcomes in patients with thin endometrium, infertility and failed assisted reproduction after administration of secretome of peripheral blood mononuclear cells in suppositories

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Abstract

Endometrial thickness (ET) <8 mm has an unfavorable impact on perinatal outcomes. One of the areas in treatment for thin endometrium is the use of cellular technologies that influence the reparative process, such as autologous platelet-rich plasma, peripheral blood mononuclear cells (PBMCs). The use of acellular secretome is a new trend with similar therapeutic properties that has been developed in regenerative medicine.

Objective: To evaluate the effect of secretome of peripheral blood mononuclear cells (SPBMC) on ET in women with thin endometrium and infertility.

Materials and methods: The study included 252 reproductive-aged infertile patients with a previous history of two or more attempts of assisted reproduction and the presence of thin endometrium not responding to therapy using other methods. In addition to the standard therapy, the patients received SPBMC Superlymph 25 units one suppository vaginally at night for 20 days from the 5th to the 25th day of the menstrual cycle from 1 to 3 months until there were satisfactory parameters of ET (8 mm or more) confirmed by ultrasound. All patients had a single cryopreserved embryo transfer performed.

Results: The statistical analysis of the data (n=252) showed a significant increase in ET to 8 mm or more in 80.2% of patients compared to the initial values. The effectiveness of assisted reproduction was as follows: the overall rate of clinical pregnancy per transfer was 38.1% (105/247), namely 47.2% (17/36) in patients aged 18–29, 39.8% (37/93) in patients aged 30–35, 38.5% (37/96) in patients aged 36–40, and 13.6% (3/22) in patients aged 41–45. The birth rate was 35.3% (36/102), namely 36.8% (7/19) in patients aged 18-29, 42.2% (19/45) in patients aged 30-35, and 27.8% (10/36) in patients aged 36-40.

Conclusion: The use of SPBMC in combination with standard therapy increases endometrial thickness, improves pregnancy and live birth rates in patients with thin endometrium and infertility.

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About the authors

Nadezhda M. Erlikhman

Istochnik IVF Clinic

Author for correspondence.
Email: nadyaerlih@mail.ru
ORCID iD: 0009-0002-7011-6888

PhD, Deputy Chief Physician, Chief IVF Specialist, Obstetrician-Gynecologist, Ultrasound specialist

Russian Federation, Chelyabinsk

Alexandr A. Yakovlev

Biotechfarm LLC

Email: nadyaerlih@mail.ru
ORCID iD: 0009-0000-3062-2159

Director for Medical Research

Russian Federation, Moscow

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