The cluster approach to assessment of the subgingival microbiome composition in women with adverse pregnancy outcomes

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Abstract

Objective: To develop optimal approach to assessment of the clinical features of the gestational process and the role of associations of the members of the subgingival microbiome in the development of pregnancy complications associated with inflammatory changes in periodontium.

Materials and methods: The total number of patients in the study was 146 pregnant women, who were divided into 4 groups depending on the presence or absence of the concomitant inflammation of periodontal tissues (gingivitis) and/or local inflammation of the lower reproductive tract. The study design included obstetric and gynecological anamneses collection, assessment of the current pregnancy complications, determination of the composition of the subgingival microbiome and the vaginal microbiome using PCR. Statistical data processing included cluster analysis.

Results: The study found relationship between systemic manifestations of periodontal diseases and preterm birth, low birth weight. There was statistically significant relationship between these complications and gingivitis. Cluster analysis was used to identify 4 different associations of the microorganisms exhibiting periodontopathogenic properties, 3 of them were found in more than half of cases in the groups with concomitant gingivitis.

Conclusion: The cluster approach to assessment of the subgingival microbiome in pregnant women helped to identify associations of the microorganisms exhibiting periodontopathogenic properties. High frequency of their occurrence was accompanied by the presence of clinical signs of gingivitis and the development of systemic effects of pregnancy complications.

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About the authors

Anna G. Burduli

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Author for correspondence.
Email: burdulianna@gmail.com
ORCID iD: 0000-0002-2849-5426

PhD, Senior Researcher at the B.V. Leonov Department of Assisted Technologies for the Treatment of Infertility

Russian Federation, 117997, Moscow, Ac. Oparin str., 4

Irina P. Balmasova

Russian University of Medicine, Ministry of Health of Russia

Email: iri.balm@mail.ru
ORCID iD: 0000-0001-8194-2419

Dr. Med. Sci., Professor, Leading Researcher at the Laboratory of Molecular Biological Research, Scientific Research Medical Dentistry Institution, Scientific and Educational Institution of Basic Medicine named after V.I. Pokrovsky

Russian Federation, 127006, Moscow, Dolgorukovskaya str. 4

Nana K. Tetruashvili

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: tetrauly@mail.ru
ORCID iD: 0000-0002-9201-2281

Dr. Med. Sci., Associate Professor, Head of the Obstetric Department of Pregnancy Pathology No. 2

Russian Federation, 117997, Moscow, Ac. Oparin str., 4

Andrey E. Donnikov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: donnikov@dna-technology.ru
ORCID iD: 0000-0003-3504-2406

PhD, Associate Professor, Head of the Laboratory of Molecular Genetic Methods

Russian Federation, 117997, Moscow, Ac. Oparin str., 4

Regina V. Krasheninnikova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: r_krasheninnikova@oparina4.ru
ORCID iD: 0009-0002-2903-1225

Geneticist at the Laboratory of Molecular Genetic Methods

Russian Federation, 117997, Moscow, Ac. Oparin str., 4

Sergey D. Arutyunov

Russian University of Medicine, Ministry of Health of Russia

Email: sd.arutyunov@mail.ru
ORCID iD: 0000-0001-6512-8724

Dr. Med. Sci., Professor, Head of Department of Orthopedic Dentistry and Digital Technologies

Russian Federation, 127473, Moscow, Delegatskaya str., 20-1

References

  1. Макеева И.М., Игнатко А.А., Чурганова А.А., Лебедев В.А., Mакеева M.K. Болезни пародонта и осложненное течение беременности. Стоматология. 2019; 98(1): 70-3. [Makeeva I.M., Ignatko A.A., Churganova A.A., Lebedev V.A., Makeeva M.K. Periodontal diseases and complicated pregnancy. Stomatology. 2019; 98(1): 70-3 (in Russian)]. https://dx.doi.org/10.17116/stomat20199801170
  2. Oliveira L.J.C., Cademartori M.G., Sfreddo C.S., Silveira M.F.D., Barros F.C., Correa M.B. et al. Factors associated with periodontal diseases in pregnancy: findings of the 2015 Pelotas birth cohort study. Braz. Oral. Res. 2023; 37: e110. https://dx.doi.org/10.1590/1807-3107bor-2023.vol37.0110
  3. Gesase N., Miranda-Rius J., Brunet-Llobet L., Lahor-Soler E., Mahande M.J., Masenga G. The association between periodontal disease and adverse pregnancy outcomes in Northern Tanzania: a cross-sectional study. Afr. Health Sci. 2018; 18(3): 601-11. https://dx.doi.org/10.4314/ahs.v18i3.18
  4. Gallardo Chávez L.M., Rodríguez Díaz J.M., Juárez Medel C.A., Hernández Clemente J., Herrera Santos A.U. Prevalence of gingivitis and risk factors among pregnant women from Acapulco, Guerrero: a cross-sectional study. Rev. Cient. Odontol. (Lima). 2022; 10(1): e094. https://dx.doi.org/10.21142/2523-2754-1001-2022-094
  5. Shrestha R., Pradhan S., Baral G. Prevalence of gingivitis in second trimester of pregnancy. Kathmandu Univ. Med. J. (KUMJ). 2022; 20(79): 301-6.
  6. Балмасова И.П., Царёв В.Н., Янушевич О.О., Маев И.В., Мкртумян М.А., Арутюнов С.Д. Микроэкология пародонта. Взаимосвязь локальных и системных эффектов. М.: Практическая медицина; 2021. 264 с. [Balmasova I.P., Tsarev V.N., Yanushevich O.O., Maev I.V., Mkrtumyan M.A., Arutyunov S.D. Periodontal microecology. Interrelation of local and systemic effects. Moscow: Practical medicine; 2021. 264 p. (in Russian)].
  7. Sanz M., Marco Del Castillo A., Jepsen S., Gonzalez-Juanatey J.R., D'Aiuto F., Bouchard P. et al. Periodontitis and cardiovascular diseases: consensus report. J. Clin. Periodontol. 2020; 47(3): 268-88. https://dx.doi.org/10.1111/jcpe.13189
  8. Walther K.A., Gröger S., Vogler J.A.H., Wöstmann B., Meyle J. Inflammation indices in association with periodontitis and cancer. Periodontol. 2000. 2024; 96(1): 281-315. https://dx.doi.org/10.1111/prd.12612
  9. Le Q.A., Akhter R., Coulton K.M., Vo N.T.N., Duong L.T.Y., Nong H.V. et al. Periodontitis and preeclampsia in pregnancy: a systematic review and meta-analysis. Matern. Child Health. J. 2022; 26(12): 2419-43. https://dx.doi.org/10.1007/s10995-022-03556-6
  10. Saadaoui M., Singh P., Khodor S.A. Oral microbiome and pregnancy: a bidirectional relationship. J. Reprod. Immunol. 2021; 145: 103293. https://dx.doi.org/10.1016/j.jri.2021.103293
  11. Гажва С.И., Сулима А.Н., Гажва Ю.В., Тетерин А.И., Кашин Ю.А. Микробиом полости рта и его влияние на течение беременности. Акушерство и гинекология. 2025; 5: 14-9. [Gazhva S.I., Sulima A.N., Gazhva Yu.V., Teterin A.I., Kashin Yu.A. Oral microbiome and its impact on pregnancy. Obstetrics and Gynecology. 2025; (5): 14-9 (in Russian)]. https://dx.doi.org/10.18565/aig.2024.332
  12. Stupak A., Kwaśniewski W. Evaluating current molecular techniques and evidence in assessing microbiome in placenta-related health and disorders in pregnancy. Biomolecules. 2023; 13(6): 911. https://dx.doi.org/10.3390/biom13060911
  13. Xu B., Han Y.W. Oral bacteria, oral health, and adverse pregnancy outcomes. Periodontol. 2000. 2022; 89(1): 181-9. https://dx.doi.org/10.1111/prd.12436
  14. Регистрационное удостоверение на медицинское изделие от 04.04.2016 г. № РЗН 2016/3903. Инструкция по применению набора реагентов для определения профиля экспрессии мРНК генов врожденного иммунитета с целью оценки локального воспаления нижних отделов женского репродуктивного тракта методом ОТ-ПЦР в режиме реального времени (ИммуноКвантэкс С/V). М.: НПО ДНК-Технология; 2015. 24 c. [Registration certificate for a medical device dated 04.04.2016, No. RZN 2016/3903. Instructions for use of a set of reagents for determining the mRNA expression profile of innate immunity genes in order to assess local inflammation of the lower parts of the female reproductive tract using real-time RT-PCR (ImmunoQuantex C/V). Moscow: NPO DNA-Technology; 2015. 24 p. (in Russian)].

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2. Figure 1. Percentage deviations of Parodontoskrin test results in pregnant women with local inflammation (groups 2–4) from those in the absence of inflammation (group 1)

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3. Figure 2. Clusters of periodontal pathogens in women with pregnancy complications potentially associated with gingivitis

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