The cluster approach to assessment of the subgingival microbiome composition in women with adverse pregnancy outcomes

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Objective: To develop optimal approach to assessment of the clinical features of the gestational process and the role of associations of the members of the subgingival microbiome in the development of pregnancy complications associated with inflammatory changes in periodontium.

Materials and methods: The total number of patients in the study was 146 pregnant women, who were divided into 4 groups depending on the presence or absence of the concomitant inflammation of periodontal tissues (gingivitis) and/or local inflammation of the lower reproductive tract. The study design included obstetric and gynecological anamneses collection, assessment of the current pregnancy complications, determination of the composition of the subgingival microbiome and the vaginal microbiome using PCR. Statistical data processing included cluster analysis.

Results: The study found relationship between systemic manifestations of periodontal diseases and preterm birth, low birth weight. There was statistically significant relationship between these complications and gingivitis. Cluster analysis was used to identify 4 different associations of the microorganisms exhibiting periodontopathogenic properties, 3 of them were found in more than half of cases in the groups with concomitant gingivitis.

Conclusion: The cluster approach to assessment of the subgingival microbiome in pregnant women helped to identify associations of the microorganisms exhibiting periodontopathogenic properties. High frequency of their occurrence was accompanied by the presence of clinical signs of gingivitis and the development of systemic effects of pregnancy complications.

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作者简介

Anna Burduli

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

编辑信件的主要联系方式.
Email: burdulianna@gmail.com
ORCID iD: 0000-0002-2849-5426

PhD, Senior Researcher at the B.V. Leonov Department of Assisted Technologies for the Treatment of Infertility

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Irina Balmasova

Russian University of Medicine, Ministry of Health of Russia

Email: iri.balm@mail.ru
ORCID iD: 0000-0001-8194-2419

Dr. Med. Sci., Professor, Leading Researcher at the Laboratory of Molecular Biological Research, Scientific Research Medical Dentistry Institution, Scientific and Educational Institution of Basic Medicine named after V.I. Pokrovsky

俄罗斯联邦, 127006, Moscow, Dolgorukovskaya str. 4

Nana Tetruashvili

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: tetrauly@mail.ru
ORCID iD: 0000-0002-9201-2281

Dr. Med. Sci., Associate Professor, Head of the Obstetric Department of Pregnancy Pathology No. 2

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Andrey Donnikov

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: donnikov@dna-technology.ru
ORCID iD: 0000-0003-3504-2406

PhD, Associate Professor, Head of the Laboratory of Molecular Genetic Methods

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Regina Krasheninnikova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: r_krasheninnikova@oparina4.ru
ORCID iD: 0009-0002-2903-1225

Geneticist at the Laboratory of Molecular Genetic Methods

俄罗斯联邦, 117997, Moscow, Ac. Oparin str., 4

Sergey Arutyunov

Russian University of Medicine, Ministry of Health of Russia

Email: sd.arutyunov@mail.ru
ORCID iD: 0000-0001-6512-8724

Dr. Med. Sci., Professor, Head of Department of Orthopedic Dentistry and Digital Technologies

俄罗斯联邦, 127473, Moscow, Delegatskaya str., 20-1

参考

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2. Figure 1. Percentage deviations of Parodontoskrin test results in pregnant women with local inflammation (groups 2–4) from those in the absence of inflammation (group 1)

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3. Figure 2. Clusters of periodontal pathogens in women with pregnancy complications potentially associated with gingivitis

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