ALLELIC POLYMORPHISM OF THE GENES OF SOME CYTOKINES IN PATIENTS WITH IN VITRO FERTILIZATION FAILURES


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Abstract

Objective. To define the importance of allelic variants of cytokine genes in the impaired processes of implantation for infertility of unknown genesis or tuboperitoneal infertility in patients with multiple in vitro fertilization (IVF) failures. Subjects and methods. The study covered 65 patients (a study group) who had undergone 2 to 5 failed IVF attempts, including 35 for primary infertility of unknown genesis and 30 for tuboperitoneal infertility. A comparison group included 70 women with realized reproductive function. In all the patients, the polymorphism of the cytokine genes IL-1ß (-511C/T), IL-6 (-174G/C), IL-10 (-1082 G/A), TNF-α (-238G/A) was studied using polymerase chain reaction- restriction fragment length polymorphism. Results. The patients with multiple failed IVF attempts were found to have a proinflammatory and antiinflammatory cytokine imbalance that manifested itself as the higher frequency of allelic variants in the genotypes of the IL-1ß and TNF-α genes. Conclusion. On examining patients with multiple IVF failures, it is appropriate to perform a molecular genetic study to reveal their predisposition to impaired implantation and, possibly, to make pregravid preparation.

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About the authors

M. V BELOKUROVA

M.V. Lomonosov Moscow State University; Center for Family Planning and Reproduction, Moscow Healthcare Department

Email: leit@mail.ru
Department of Obstetrics and Gynecology, Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

L. M SAMOKHODSKAYA

M.V. Lomonosov Moscow State University; Center for Family Planning and Reproduction, Moscow Healthcare Department

Department of Obstetrics and Gynecology, Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

M. P KRAMARENKO

M.V. Lomonosov Moscow State University; Center for Family Planning and Reproduction, Moscow Healthcare Department

Department of Obstetrics and Gynecology, Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

O. N SADEKOVA

M.V. Lomonosov Moscow State University; Center for Family Planning and Reproduction, Moscow Healthcare Department

Department of Obstetrics and Gynecology, Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

O. B PANINA

M.V. Lomonosov Moscow State University; Center for Family Planning and Reproduction, Moscow Healthcare Department

Department of Obstetrics and Gynecology, Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

G. M SAVELYEVA

M.V. Lomonosov Moscow State University; Center for Family Planning and Reproduction, Moscow Healthcare Department

Department of Obstetrics and Gynecology, Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

V. A TKACHUK

M.V. Lomonosov Moscow State University; Center for Family Planning and Reproduction, Moscow Healthcare Department

Department of Obstetrics and Gynecology, Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

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