STUDY OF ENDOMETRIAL RECEPTIVITY MARKERS IN CERVICAL MUCUS AS A NONINVASIVE METHOD FOR ESTIMATING THE IMPLANTATION POTENTIAL
- Авторлар: Seidova L.A.1, Perminova S.G.1, Demura T.A.1
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Мекемелер:
- Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
- Шығарылым: № 5 (2015)
- Беттер: 74-79
- Бөлім: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/247421
- ID: 247421
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Аннотация
Objective. To evaluate endometrial receptivity in infertile patients, by measuring the cervical mucus (CM) concentrations of leukemia inhibitory factor (LIF) and its receptor (LIF-R) and comparing with their immunohistochemical expression in endometrial tissue. Subjects and methods. Sixty patients aged 26 to 38 years with tuboperitoneal factor infertility who had a history of more than two in vitro fertilization (IVF) failures (a study group) and 20 healthy fertile women of the same age (a control group) were examined. Both groups of women underwent measurement of CM LIF and LIF-R concentrations by enzyme immunoassay, morphological examination of the endometrium, by identifying mature pinopidia, and its immunohistochemical examination, by estimating LIF and LIF-R expression in the implantation window of a natural cycle. Results. In the study group, the CM concentration of LIF was significantly lower than that in the control group on days 3 and 7 after urine luteinizing hormone (LH) surge (4.9±3.4 and 73±2.8 pg/ml, respectively; р < 0.05 and 27.2±9.6 and 32.2+7.3 pg/ml; р < 0.05) and that of LIF-R was also significantly lower than that in the control group on days 5 (113.0+37.4 and 156.0+18.7 pg/ml, р < 0.05), 7 (174.2+42.4 and 210.0+6.7 pg/ml, р < 0.05), and 9 (52.0+10.6 and 98.1+25.3 pg/ml, р < 0.05) after urine LH surge. Endometrial immunohistochemical examination revealed the diminished expression of LIF (4.4+0.2 and 6.0+0.1 scores, р <0.05) and LIF-R (4.2+0.4 and 6.0+0.2 scores, р < 0.05) in the study group as compared to the control one. A moderate direct correlation was found between the CM concentration of markers and the level of their expression in the endometrium: LIF (r = 0.67), LIF-R (r = 0.64). Conclusion. The CM concentrations of LIF and LIF-R and their immunohistochemical expression in the endometrium were decreased in infertile women with a history of repeated IVF failures. Determination of CM LIF and LIF-R concentrations may be used as a noninvasive method to estimate the implantation potential of the endometrium.
Толық мәтін
Авторлар туралы
Laman Seidova
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: lamacfar@gmail.com
a graduate student Moscow 117997, Ac. Oparina str. 4, Russia
Svetlana Perminova
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: perisvet@list.ru
MD, Leading Researcher, 1st gynecological department Moscow 117997, Ac. Oparina str. 4, Russia
Tatyana Demura
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: demura-t@yandex.ru
Candidate of medical sciences, senior researcher at the 1st Pathology Department Moscow 117997, Ac. Oparina str. 4, Russia
Әдебиет тізімі
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