THE DETECTION RATE AND THE STRUCTURE OF PATHOLOGICAL CHANGES OF THE ENDOMETRIUM IN WOMEN OF REPRODUCTIVE AGE WITH ENDOMETRIOSIS


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Objective. To determine the frequency of various pathological changes in the endometrium of reproductive-aged women with endometriosis Subjects and methods. The endometrium was sampled from 236 patients: 118 women with endometriosis and 118 without this condition (a comparison group) and morphologically examined by the conventional procedure. Immunohistochemical (IHC) examination used monoclonal antibodies against CD 138 (RTU, clone D0-7, Dako, Denmark) and PTEN (clone 6H2.1, dilution 1:100). The Dako REAL EnVision Detection system (Dako, Denmark) was used as secondary antibodies. To visualize antibody-antigen binding sites, the investigators applied the oxidation reaction of 3,3-diaminobenzidine by horseradish peroxidase in the presence of hydrogen peroxide to form a water-insoluble brown end product. After carrying out IHC reactions, the sections were counterstained with hematoxylin and mounted using the Shandon mount™ synthetic medium (USA). Negative and positive controls were used to correctly carry out IHC tests. Results. The level of pathological changes in the endometrium was considerably higher in the endometriosis group than that in the non-endometriosis one (50% vs 27.1%). Chronic endometritis was diagnosed in 22.03% (26/118) of cases in the endometriosis group and in 11.02% (10/118) in the non-endometriosis group. Endometrial polyp was detected in 17.8% (21/118) of cases among the patients with endometriosis and in 8.47% (10/118) among those in the comparison group. Endometrial hyperplasia was diagnosed in 10.17% (12/118) in the patients with endometriosis and in 7.63% (9/118) among those in the comparison group. However, there were no statistically significant differences in the frequency of pathological changes in the endometrium between the early (I+II) and late (III+IV) stages of endometriosis. Conclusion. Our study has shown that the frequency of pathological changes, such as chronic endometritis and endometrial polyp, is higher among the patients with genital endometriosis than among those without this disease. The emergence of endometrioid foci is closely related to pathological changes in the endometrium; however, the sequence of occurrence of pathological changes in the uterus and small pelvic cavity remains unclear and will be the subject of our further investigations.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Ekaterina Tikhonchuk

Email: best-kauty@mail.ru
PhD-student, Department of Surgical Gynecology, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 119997, Russia, Moscow, Ac. Oparina str. 4

Alexandra Asaturova

Email: a_asaturova@oparina4.ru
PhD, senior scientific researcher of pathology department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia 119997, Russia, Moscow, Ac. Oparina str. 4

Leila Adamyan

Email: l_adamyan@oparina4.ru
MD, professor, academician of RAS; deputy director on science, head of department of operative gynecology, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 119997, Russia, Moscow, Ac. Oparina str. 4

Әдебиет тізімі

  1. Адамян Л.В., Андреева Е.Н., Аполихина И.А., Беженарь В.Ф., Геворкян М.А., Гус А.И., Демидов В.Н., Калинина Е.А., Леваков С.А., Марченко Л.А., Попов А.А., Сонова М.М., Ханукоева А.З., Чернуха Г.Е., Яроцкая Е.Л. Эндометриоз: диагностика, лечение и реабилитация. Федеральные клинические рекомендации по ведению больных. М.: Российское общество акушеров-гинекологов; 2013. 65с. [Adamyan L.V., Andreeva E.N., Apolikhina I.A., Bezhenar V.F., Gevorkyan M.A, Gus A.I., Demidov V.N., Kalinina E.A., Levakov S.A., Marchenko L.A., Popov A.A., Sonova M.M., Khanukoeva A.Z., Chernukha G.E., Yarotskaya E.L. Endometriosis: Diagnosis, treatment and rehabilitation. Federal guidelines for the management of patients. Moscow: Russian Society of Obstetricians and Gynecologists; 2013. 65p. (in Russian)]
  2. Адамян Л.В., Спицын В.А., Андреева Е.Н. Генетические аспекты гинекологических заболеваний. М.: ГЭОТАР-Медиа; 2008. 215с. [Adamyan L.V., Spitsyn V.A. Andreeva E.N. Genetic aspects of gynecological diseases. Moscow: GEOTAR-Media; 2008. 215p. (in Russian)]
  3. Адамян Л.В. Эндометриозы. Руководство для врачей. M.: Медицина; 1998. 320с. [Adamyan L.V. Endometriosis. Guidelines for doctors. Moscow: Meditsina; 1998. 320p. (in Russian)]
  4. Адамян Л.В., Азнаурова Я.Б. Молекулярные аспекты патогенеза эндометриоза. Проблемы репродукции. 2015; 21(2): 67-77. [Adamyan L.V., Aznaurova Ya.B. Molecular aspects of the pathogenesis of endometriosis. Problemy Reproduktsii (Russian Journal of Human Reproduction). 2015; 21(2): 67-77. (in Russian)]
  5. Тихончук Е.Ю., Асатурова А.В., Адамян Л.В. Молекулярно-биологические изменения эндометрия у женщин с наружным генитальным эндометриозом. Акушерство и гинекология. 2016; 11: 42-8. http://dx.doi.org/10.18565/ aig.2016.11.42-8 [Tikhonchuk E.Yu., Asaturova A.V., Adamyan L.V. Endometrial molecular biological changes in women with external genital endometriosis. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2016; (11): 42-8. (in Russian) http://dx.doi.org/10.18565/aig.2016.11.42-8]
  6. Зайратьянц О.В., Адамян Л.В., Андреева Е.Н., Максимова Ю.В., Мурдалова З.Х., Опаленов К.В., Мовтаева Х.Р., Сонова М.М. Молекулярно-биологические особенности эктопического и эутопического эндометрия при генитальном эндометриозе. Архив патологии. 2010; 72(5): 6-12. [Zayratyants O.V., Adamyan L.V., Andreeva E.N., Maksimova Yu.V., Murdalova Z.H., Opalenov K.V., Movtaeva H.R., Sonova M.M. Molecular-biological features of ectopic and eutopic endometrium in genital endometriosis. Arkhiv patologii. 2010; 72(5): 6-12. (in Russian)]
  7. Зайратьянц О.В., Адамян Л.В., Сомова М.М., Борзенкова И.П., Опаленов К.В., Барсанова Т.Г., Мовтаева Х.Р. Экспрессия ароматазы цитохрома Р-450 в эктопическом и эутопическом эндометрии при эндометриозе. Архив патологии. 2008; 70(5): 16-20. [Zayratyants O.V., Adamyan L.V., Somova M.M., Borzenkova I.P., Opalenov K.V., Barsanova T.G., Movtaeva X.R. The expression of aromatase cytochrome P-450 in ectopic and eutopic endometrium of endometriosis. Arkhiv patologii. 2008; 70(5): 16-20. (in Russian)]
  8. Тихончук Е.Ю., Непша О.С., Адамян Л.В., Кузнецова М.В. Омиксные технологии в исследовании патогенеза эндометриоза (обзор литературы). Проблемы репродукции. 2016; 22(5): 110-22. doi: 10.17116/repro2016225110-122 [Tihonchuk E.Yu., Nepsha O.S., Adamyan L.V., Kuznetsova M.V. ‘Omic’ high-throughput technologies in research on pathogenesis of endometriosis (a review). Problemy Reproduktsii (Russian Journal of Human Reproduction). 2016; 22(5): 110-22. (in Russian) doi: 10.17116/repro2016225110-122]
  9. Sourial S., Tempest N., Hapangama D.K. Theories on the pathogenesis of endometriosis. Int. J. Reprod. Med. 2014;2014:179515. doi: 10.1155/2014/179515.
  10. Takebayashi A., Kimura F., Kishi Y., Ishida M., Takahashi A., Yamanaka A. et al. The association between endometriosis and chronic endometritis. PLoS One. 2014; 9(2): e88354. doi: 10.1371/journal.pone.0088354. eCollection 2014.
  11. Berbic M., Schulke L., Markham R., Tokushige N., Russell P., Fraser I.S. Macrophage expression in endometrium of women with and without endometriosis. Hum. Reprod. 2009; 24(2): 325-32. doi: 10.1093/humrep/ den393.
  12. Siristatidis C., Nissotakis C., Chrelias C., Iacovidou H., Salamalekis E. Immunological factors and their role in the genesis and development of endometriosis. J. Obstet. Gynaecol. Res. 2006; 32(2): 162-70.
  13. Cicinelli E., Resta L., Nicoletti R., Tartagni M., Marinaccio M., Bulletti C., Colafiglio G. Detection of chronic endometritis at fluid hysteroscopy. J. Minim. Invasive Gynecol. 2005; 12(6): 514-8.
  14. Kitaya K. Prevalence of chronic endometritis in recurrent miscarriages. Fertil. Steril. 2011; 95(3): 1156-8. doi: 10.1016/j.fertnstert.2010.09.061.
  15. Johnston-MacAnanny E.B., Hartnett J., Engmann L.L., Nulsen J.C., Sanders M.M., Benadiva C.A. Chronic endometritis is a frequent finding in women with recurrent implantation failure after in vitro fertilization. Fertil. Steril. 2010; 93(2): 437-41. doi: 10.1016/j.fertnstert.2008.12.131.
  16. Nasu K., Yuge A., Tsuno A., Nishida M., Narahara H. Involvement of resistance to apoptosis in the pathogenesis of endometriosis. Histol. Histopathol. 2009; 24(9): 1181-92.
  17. Tong J.L., Lang J.H., Zhu L., Feng R.E., Cui Q.C. Study on pathological characteristics of eutopic endometrium in endometriosis. Zhonghua Fu Chan Ke Za Zhi. 2012; 47(11): 829-32.
  18. Kitaya K., Yasuo T. Immunohistochemistrical and clinicopathological characterization of chronic endometritis. Am. J. Reprod. Immunol. 2011; 66(5): 410-5. doi: 10.1111/j.1600-0897.2011.01051.x.
  19. Kasius J.C., Broekmans F.J., Sie-Go D.M., Bourgain C., Eijkemans M.J., Fauser B. C. et al. The reliability of the histological diagnosis of endometritis in asymptomatic IVF cases: a multicenter observer study. Hum. Reprod. 2012; 27(1): 153-8. doi: 10.1093/humrep/der341.
  20. Zheng Q.M., Mao H.I., Zhao Y.J., Zhao J., Wei X., Liu P.S. Risk of endometrial polyps in women with endometriosis: a meta-analysis. Reprod. Biol. Endocrinol. 2015; 13: 103. doi: 10.1186/s12958-015-0092-2.
  21. Shen L., Wang Q., Huang W., Wang Q., Yuan Q., Huang Y., Lei H. High prevalence of endometrial polyps in endometriosis-associated infertility. Fertil. Steril. 2011; 95(8): 2722-4. e1. doi: 10.1016/j.fertnstert.2011.04.067.
  22. Indraccolo U., Di Iorio R., Matteo M., Corona G., Greco P., Indraccolo S.R. The pathogenesis of endometrial polyps: a systematic semi-quantitative review. Eur. J. Gynaecol. Oncol. 2013; 34: 5-22.
  23. Kitawaki J., Kado N., Ishihara H., Koshiba H., Kitaoka Y., Honjo H. Endometriosis: the pathophysiology as an estrogen-dependent disease. J. Steroid Biochem. Mol. Biol. 2002; 83: 149-55.
  24. Park J.S., Lee J.H., Kim M., Chang H.J., Hwang K.J., Chang K.H. Endometrium from women with endometriosis shows increased proliferation activity. Fertil Steril. 2009; 92(4): 1246-9. doi: 10.1016/j.fertnstert.2009.04.025.
  25. Dmowski W.P., Ding J., Shen J., Rana N., Fernandez B.B., Braun D.P. Apoptosis in endometrial glandular and stromal cells in women with and without endometriosis. Hum. Reprod. 2001; 16(9): 1802-8.
  26. Harada T., Taniguchi F., Izawa M., Ohama Y., Takenaka Y., Tagashira Y. et al. Apoptosis and endometriosis. Front. Biosci. 2007; 12: 3140-51.
  27. Omwandho C.O., Konrad L., Halis G., Oehmke F., Tinneberg H.R. Role of TGF-betas in normal human endometrium and endometriosis. Hum. Reprod. 2010; 25: 101-9.
  28. Mantovani A., Allavena P., Sica A., Balkwill F. Cancer-related inflammation. Nature. 2008; 454(7203): 436-44. doi: 10.1038/ nature07205.
  29. Porta C., Riboldi E., Sica A. Mechanisms linking pathogens-associated inflammation and cancer. Cancer Lett. 2011; 305(2): 250-62. doi: 10.1016/j. canlet.2010.10.012.
  30. Pikarsky E., Porat R.M., Stein I., Abramovitch R., Amit S., Kasem S. et al. NF-kappaB functions as a tumour promoter in inflammation-associated cancer. Nature. 2004; 431(7007): 461-6.

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