Somatic mutations in MED 12 gene exon 2 in women with a single uterine fibroid or multiple ones


Дәйексөз келтіру

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Аннотация

Objective. To search for genetic markers for uterine fibroids in order to optimize diagnosis and management tactics in patients and to predict the risk of recurrence. Subjects and methods. Tissue samples from 167 fibroids and blood aliquots from 65 patients were collected. DNA was extracted and MED12 gene exon 2 amplified. Polymerase chain reaction was carried out to determine sequences using the Sanger method. Results. Women with compromised and uncompromised histories showed different somatic mutation ratios in MED12 gene exon 2 and differences in the distribution of mutations when comparing women with a single uterine fibroid or multiple ones. Conclusion. The findings could reveal a possible interaction of a mutation in the gene under study with a compromised history. Somatic mutation saturation in the subgroups of patients with multiple myomatous nodules indicates that multiple uterine fibroids mainly arise from MED 12 gene mutations, which may be less typical for single f ibro ids.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Nelly Sogoyan

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: sogoyan.n@mail.ru
resident

Maria Kuznetsova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: mkarja@mail.ru
PhD, research scientist, laboratory of molecular-genetic methods

Alexanda Asaturova

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_asaturova@oparina4.ru
PhD, pathologist, senior scientific assistant

Leila Adamyan

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia; Moscow State University of Medicine and Dentistry

Email: l_adamyan@oparina4.ru
Academician of RAS, MD, PhD, Professor RAS, Honored Master of Science of the Russian Federation, Head Specialist in Obstetrics and Gynecology of Ministry of Healthcare of Russia, Head of the Department of Surgical Gynecology; Head of the Department of Reproductive Medicine and Surgery, Faculty of Postgraduate Education

Dmitry Trofimov

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

DSci, professor RAS, Head of Laboratory of molecular-genetic methods

Әдебиет тізімі

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