Association of blood vitamin K level and polymorphism of detoxification genes with the outcomes of an assisted reproductive technology program

Objective. To search for biochemical and molecular genetic predictors of pregnancy in an IVF/ICSI program. Subjects and methods. The investigation enrolled 100 women who were eligible for inclusion. In accordance with the results of infertility treatment, the investigators formed two study groups: 1) 28 patients with the onset of clinical pregnancy; 2) 72 patients with a negative treatment result. All the women underwent determination of the serum level of vitamin K and the polymorphism of the VKORC1 gene and the genes involved in xenobiotic metabolism. Results. In patients who had class C embryos (low- or poor-quality embryos) according to the classification proposed by D. Gardner, the serum level of vitamin K was lower than in those with good-quality embryos. The probability of obtaining immature oocytes for female carriers of slow CYP2C9*2 and CYP2C9*3 haplotypes was 61 and 46%, respectively, whereas immature oocytes were obtained for patients with normal genotype (*1/*1) in 72% of cases. A correlation was also found between the probability of pregnancy in the IVF/ICSI program and the EPHX1337 T>C (Tyr113His) rs1051740genotype. Conclusion. Serum vitamin Klevels in women receiving infertility treatment in the IVF/ICSI program was shown to be of prognostic value for the quality of embryos. The polymorphism of the detoxification genes (CYP2C9 and EPHX1) was found to have a significant impact on the quality of oocytes and the chance of getting pregnant, which can be a prognostic criterion for assessing the quality of embryos.

VKORC1, POSTN, EPHX1, CYP2C9, assisted reproductive technology, infertility, vitamin K, VKORC1, in vitro fertilization, periostin, POSTN, embryo implantation, EPHX1, CYP2C9