Characteristics of methylation of HOXA10 and HOXA11 genes in patients with tubal and peritoneal factor infertility and previous unsuccessful IVF attempts


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Objective. To assess methylation of HOXA10 and HOXA11 gene promoters in patients with tubal and peritoneal factor infertility and previous unsuccessful in vitro fertilization (IVF) attempts. Materials and methods. Before starting the IVF program 47 patients were performed pippele endometrial biopsy with the assessment of methylation of HOXA10 and HOXA11 gene promoters. According to the outcomes of the IVF program following the pipette biopsy, the patients were divided into two groups: group 1 included the women who achieved pregnancy, group 2 included the patients without pregnancy. Results. During the study period, the analysis of methylation of 23 CpG islands located in the promoter regions of the HOXA10 gene was performed. In the study groups statistically significant difference in methylation was not revealed in any of CpG islands of HOXA10 and HOXA11. Conclusion. The obtained results suggest that methylation of the HOXA10 and HOXA11 genes is rather a conservative parameter and it does not appear to influence the outcome of the IVFprogram in the women of the study group.

Толық мәтін

Рұқсат жабық

Авторлар туралы

E. Knyazeva

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: dr.knyazeva.ea@gmail.com
Post-graduate student of B.V. Leonov Department of Auxiliary Technologies in Infertility Treatment 4 Oparina str., Moscow, 117997, Russian Federation

M. Kuznetsova

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: mkarja@mail.ru
PhD, Researcher of the laboratory of molecular genetics methods 4 Oparina str., Moscow, 117997, Russian Federation

E. Shubina

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_shubina@oparina4.ru
PhD, head of the Department of Laboratory genomic data 4 Oparina str., Moscow, 117997, Russian Federation

A. Goltsov

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: andrey.goltsov@gmail.com
Researcher of the laboratory of molecular genetics methods 4 Oparina str., Moscow, 117997, Russian Federation

A. Donnikov

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
PhD, Head of the Department of Laboratory and Genetic Methods 4 Oparina str., Moscow, 117997, Russian Federation

E. Kalinina

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
MD, Associate Professor, Chief of B.V. Leonov Department of Auxiliary Technologies in Infertility Treatment named after professor B.V. Leonov 4 Oparina str., Moscow, 117997, Russian Federation

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