Influence of controlled mechanical microvibration on the oocyte fertilization and embryo during the first five days of development


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Objective. To evaluate the influence of controlled mechanical microvibration on oocyte fertilization rate and embryo during the first five days of development. Materials and methods. The quality of 952 embryos obtained from 166 patients was evaluated in the microvibration group. The quality of3369 embryos obtained from 757patients was assessed in the control group. In the microvibration group, the incubator was placed on the ArisTT180-s platform (K&S Advanced Systems Ltd, Israel) in the active vibration mode with a frequency of 40 Hz for 30 seconds with intervals of 30 minutes. Results. The number of embryos scored as grades one and five (according to Gardner’s classification), with grades AA and BA increased during the cultivation under the conditions of controlled mechanical microvibration. The rate of embryo cryopreservation was 1.22 times higher in the microvibration group in comparison with the control group. The average number of cryopreserved embryos was 1 (0 - 3) in patients of the microvibration group and 0 (0 - 2) in patients of the control group (p=0.003). Conclusion. Controlled mechanical microvibration can be used in oocyte fertilization and cultivation of embryos to increase the quality of embryos on the fifth day of cultivation and to improve the possibility of obtaining embryos suitable for cryopreservation.

Негізгі сөздер

Толық мәтін

Рұқсат жабық

Авторлар туралы

Andrey Romanov

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: romanov1553@yandex.ru
postgraduate student, researcher of R&D Department

Evgeniy Romanov

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_romanov@oparina4.ru
embryologist of IVF Department

Nataliya Makarova

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: np_makarova@oparina4.ru
Ph.D., Researcher of ART Department

Nataliya Dolgushina

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_dolgushina@oparina4.ru
Dr. Med. Sci., Deputy Director - Head of the Department of Research Administration

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