Sialidase activity of vaginal bacteria in reproductive-age women


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Bacterial sialidase activity catalyzes the degradation of protective mucosal barriers. Objective: To investigate sialidase activity of vaginal bacteria and identify sialidase genotypes and phenotypes of different species of Gardnerella spp. Materials and methods: A semiquantitative sialidase assay was developed and used to test 89 strains of vaginal bacteria, including 19 strains of Gardnerella spp. The Gardnerella spp. species were identified by DNA sequencing. Gardnerella spp. sialidase genes were detected by PCR. Results: Sialidase activity was detected in 8 strains belonging to three genera, including Anaerococcus (n=1), Bif idobacterium (n=1), and Gardnerella (n=6). G. vaginalis represented the majority of the Gardnerella strains (n=16, 84%); the proportion of G. swidsinskii was (n=3, 16%). Nine strains of G. vaginalis had only the nanH1 gene and all of these strains did not exhibit sialidase activity. The remaining seven strains of G. vaginalis simultaneously had the nanH1 and nanH3 genes, and sialidase activity was registered in 6 strains. No sialidase genes were detected in any G. swidsinskii strain. Conclusion: Most cultured species of vaginal bacteria, including lactobacilli, do not produce sialidase activity. The predominant species of the genus Gardnerella is G. vaginalis (84%); the proportion of G. swidsinskii is 16%. Bifidobacterium bifidum, Anaerococcus tetradius strains and most strains of G. vaginalis, whose genome contains both the nanH1 and nanH3 genes, can degrade sialoglicans. G. swidsinskii strains, as well as G. vaginalis strains with only the nanH1 gene, do not exhibit sialidase activity.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Elena Shipitsyna

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: shipitsyna@inbox.ru
Dr. Bio. Sci., Leading Researcher, International Department

Sofia Korkina

St. Petersburg State University; D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: ksa53@outlook.com
student at the Department of Microbiology; performing final qualification work at the Department of Medical Microbiology

Anna Krysanova

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: krusanova.anna@mail.ru
Researcher, Department of Medical Microbiology; Assistant, Department of Clinical Laboratory Diagnostics

Kseniya Kolousova

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology; St. Petersburg State University; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: gimkolos@gmail.com
student at the Department of Microbiology; performing final qualification work in the Group of Experimental Microbiology

Kira Shalepo

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: 2474l5l@mail.ru
PhD (Bio), Senior Researcher, Department of Medical Microbiology; Associate Professor, Department of Clinical Laboratory Diagnostics

Olga Budilovskaya

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: o.budilovskaya@gmail.com
Researcher, Department of Medical Microbiology; Assistant, Department of Clinical Laboratory Diagnostics

Tatiana Khusnutdinova

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: husnutdinovat@yandex.ru
Researcher, Department of Medical Microbiology; Assistant, Department of Clinical Laboratory Diagnostics

Alevtina Savicheva

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia

Email: savitcheva@mail.ru
Head of the Department of Clinical Laboratory Diagnostics

Igor Kogan

D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology

Email: ovr@ott.ru
Corresponding Member of the RAS, Dr. Med. Sci., Professor, Director

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