Placental inflammatory changes associated with mRNA expression of transcription factor genes in term pregnancy


Дәйексөз келтіру

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Аннотация

Objective: To investigate the mRNA expression levels of transcription factor genes (GATA3, RORC2, FOXP3) in the cervical canal, placenta, and fetal membranes in term gestation depending on the presence of histologic chorioamnionitis. Materials and methods: The expression of GATA3, RORC2, and FOXP3 mRNAs was detected in 42pregnant women with a healthy pregnancy who underwent a planned cesarean section for fetal malpresentation and/or uterine scar at 37-41 weeks' gestation. The analysis was performed using RT-PCR and a morphological study of placentas. Results: Histologic chorioamnionitis was detected in 28 (66.7%) patients without clinical manifestations. In the presence of funiculitis/funisitis (7.1%), there was a 10-fold increase in the expression of GATA3 mRNA in the placenta and a 4-fold decrease in the fetal membranes. Parietal chorioamnionitis (64.3%) was associated with a 2-fold increase in expression levels of RORC2 FOXP3 in the placenta. Leukocyte infiltration of the fetal membranes (26.1%) was associated with a 10-fold decrease in the expression of GATA3 mRNA in the cervical canal. Conclusion: The present study showed that more than half of women with a healthy term pregnancy had histological chorioamnionitis. Analysis of the expression of transforming factors GATA3, RORC2, FOXP3 represents a promising direction in diagnosing placental and fetal membranes inflammatory process.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Maria Kaganova

Samara State Medical University, Ministry of Health of Russia

Email: mkaganova@yandex.ru
Ph.D., Associate Professor at the Department of Obstetrics and Gynecology, Institute for Postgraduate Education

Natalia Spiridonova

Samara State Medical University, Ministry of Health of Russia

Email: nvspiridonova@mail.ru
Dr. Med. Sci., Professor, Head of the Department of Obstetrics and Gynecology, Institute for Postgraduate Education

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