Outcomes of assisted reproductive technology programs in patients with oocyte dysmorphisms
- Autores: Syrkasheva A.G.1, Dolgushina N.V.1, Makarova N.P.1, Agarsheva M.V.2, Kovalskaya E.V.1
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Afiliações:
- Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
- I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
- Edição: Nº 7 (2015)
- Páginas: 56-62
- Seção: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/247491
- ID: 247491
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Resumo
Objective. To evaluate the impact of different oocyte dysmorphisms on the outcomes of in vitro fertilization/ intracytoplasmic sperm injection programs. Subjects and methods. A prospective cohort study enrolled 343 patients treated with assisted reproductive technology (ART) programs. According to the presence or absence of oocyte dysmorphisms, the patients were divided into 3 groups: 1) women with cytoplasmic oocyte dysmorphisms; 2) those with extracytoplasmic oocyte dysmorphisms; 3) those with morphologically normal oocytes (a control group). The primary endpoint was an adjusted odds ratio (OR) for the onset of a clinical pregnancy in the examined groups. Results. The rate of ovum fertilization varied greatly among the groups and was minimal in the presence of cytoplasmic dysmorphisms. The greatest number of high-quality embryos was retrieved from the women without oocyte dysmorphisms and the maximum number of Class C embryos that stopped developing was from the patients with cytoplasmic dysmorphisms. The adjusted OR for the onset of a pregnancy among the groups was 1.37 (95% CI: 1.02-1.85) and that in the women with cytoplasmic dysmorphisms as compared to the control group was 3.69 (95% CI: 2.12-6.14). Conclusion. The quality of oocytes plays an important role in fertilization and yield of high-quality of embryos and hence the efficiency of ART programs.
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Sobre autores
Anastasia Syrkasheva
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: anast.syrkasheva@gmail.com
M.D., Postgraduate of the Department of assistive reproductive technology in the treatment of infertility
Nataliya Dolgushina
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: n_dolgushina@oparina4.ru
M.D., Ph.D., M.P.H., Head of R&D Department
Nataliya Makarova
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: np_makarova@oparina4.ru
PhD, Researcher of the Department of assistive reproductive technology in the treatment of infertility
Margarita Agarsheva
I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia
Email: agarsheva.m@gmail.com
student
Evgeniya Kovalskaya
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: e_kovalskaya@oparina4.ru
embryologist of the Department of assistive reproductive technology in the treatment of infertility
Bibliografia
- Rienzi L., Ubaldi F., Iacobelli M., Minasi M.G., Ferrero S., Sapienza F. et al. Significance of morphological attributes of the early embryo. Reprod. Biomed. Online. 2005; 10(5): 669-81.
- Ubaldi F., Rienzi L. Morphological selection of gametes. Placenta. 2008; 29 (Suppl. B): 115-20.
- Ten J., Mendiola J., de Juan J., Bernabeu R. Donor oocyte dysmorphisms and their influence on fertilization and embryo quality. Reprod. Biomed. Online. 2007; 14(1): 40-8.
- Макарова Н.П., КазарянЛ.М., Калинина Е.А., Баранова Г.Б., Поляков В.Ю. Грубозернистая деструкция цитоплазмы ооцитов в цикле экстракорпорального оплодотворения: ультраструктурный анализ. Акушерство и гинекология. 2012; 8-2: 103-5.
- Rienzi L., Ubaldi F.M., Iacobelli M., Minasi M.G., Romano S., Ferrero S. et al. Significance of metaphase II human oocyte morphology on ICSI outcome. Fertil. Steril. 2008; 90(5): 1692-700.
- Wilding M., Di Matteo L., Andretti S.D., Montanaro N., Capobianco C., Dale B. An oocyte score for use in assisted reproduction. J. Assist. Reprod. Genet. 2007; 24(8): 350-8.
- Макарова Н.П., Калинина Е.А. Критерии оценки качества ооцита в циклах ИКСИ: взгляд клинического эмбриолога. Гинекология. 2012; 14(3): 24-8. [Makarova N.P., Kalinina E.A. The criteria for assessing the quality of the oocyte in the ICSI cycles: a view of the clinical embryologist. Ginekologiya. 2012; 14 (3): 24-8. (in Russian)]
- Приказ Минздрава России №107н от 30 августа 2012 г. О порядке использования вспомогательных репродуктивных технологий, противопоказаниях и ограничениях к их применению. [Order of the Russian Ministry of Health №107n on August 30, 2012 On the order of use of assisted reproductive technologies, contraindications and limitations to their use. (in Russian)]
- Serhal P.F., Ranieri D.M., Kinis A., Marchant S., Davies M., Khadum I.M. Oocyte morphology predicts outcome of intracytoplasmic sperm injection. Hum. Reprod. 1997; 12(6): 1267-70.
- Otsuki J., Nagai Y., Chiba K. Lipofuscin bodies in human oocytes as an indicator of oocyte quality. J. Assist. Reprod. Genet. 2007; 24(7): 263-70.
- Otsuki J., Okada A., Morimoto K., Nagai Y., Kubo H. The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. Hum. Reprod. 2004; 19(7): 1591-7.
- Bell C.E., Calder M.D., Watson A.J. Genomic RNA profiling and the programme controlling preimplantation mammalian development. Mol. Hum. Reprod. 2008; 14(12): 691-701.
- Kahraman S., Yakin K., Dönmez E., Samli H., Bahçe M., Cengiz G. et al. Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Hum. Reprod. 2000; 15(11): 2390-3.
- Schmutzler A.G., Acar-Perk B., Weimer J., Salmassi A., Sievers K., Tobler M. et al. Oocyte morphology on day 0 correlates with aneuploidy as detected by polar body biopsy and FISH. Arch. Gynecol. Obstet. 2014; 289(2): 445-50.
- Lasiene K., Vitkus A., Valanciūte A., Lasys V. Morphological criteria of oocyte quality. Medicina (Kaunas). 2009; 45(7): 509-15.
- Chamayou S., Ragolia C., Alecci C., Storaci G., Maglia E., Russo E., Guglielmino A. Meiotic spindle presence and oocyte morphology do not predict clinical ICSI outcomes: a study of 967 transferred embryos. Reprod. Biomed. Online. 2006; 13(5): 661-7.
- Balaban B., Ata B., Isiklar A., Yakin K., Urman B. Severe cytoplasmic abnormalities of the oocyte decrease cryosurvival and subsequent embryonic development of cryopreserved embryos. Hum. Reprod. 2008; 23(8): 1778-85.
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