The first Russian experience with controlled mechanical microvibration in growing human embryos in assisted reproductive technology programs
- Autores: Romanov A.Y.1, Frolova A.M.1, Makarova N.P.1, Dolgushina N.V.1
-
Afiliações:
- Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
- Edição: Nº 12 (2019)
- Páginas: 120-125
- Seção: Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/248699
- DOI: https://doi.org/10.18565/aig.2019.12.120-125
- ID: 248699
Citar
Texto integral
![Acesso aberto](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_open.png)
![Acesso é fechado](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_unlock.png)
![Acesso é fechado](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_lock.png)
Resumo
Objective. To evaluate the impact of controlled mechanical microvibration of human embryos during the first five days of development on pregnancy rates in assisted reproductive technology (ART) programs. Subjects and methods. The investigation enrolled 75 (a microvibration group) and 300 (a control group) married couples without contraindications or complications developed during ART programs. During cultivation under mechanical microvibration, the incubator was placed on an ArisTT180-s platform (K&S Advanced Systems Ltd, Israel) in the active vibration mode with a frequency of 40 Hz for 30 seconds at a rest interval of 30 minutes. Results. The pregnancy rate per ovarian stimulation cycle was 42.7% (n = 32) in the microvibration group versus 33.7% (n = 101) in the control group (p = 0.09). The pregnancy rate per embryo transfer was 50.8 and 40%, respectively (p = 0.097). Conclusion. The use of controlled mechanical microvibration to grow embryos can significantly increase pregnancy rates in ART programs.
Palavras-chave
Texto integral
![Acesso é fechado](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_lock.png)
Sobre autores
Andrey Romanov
Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: romanovl553@yandex.ru
Alexandra Frolova
Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: i.a.m.frolova@mail.ru
Nataliya Makarova
Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: np_makarova@oparina4.ru
Nataliya Dolgushina
Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia
Email: n_dolgushina@oparina4.ru
Bibliografia
- Shafei R.A., Syrkasheva A.G., Romanov A.Y., Makarova N.P., Dolgushina N. V., Semenova M.L. Blastocyst hatching in humans. Russ J Dev Biol. 2017; 48(1): 5-15. PMID: 30272915
- Романов А.Ю., Ковальская Е.В., Мака/ова Н.П., Сы/кашева А.Г., Долгушина Н.В. Использование цейтраферной съемки для оценки качества эмбрионов человека в программах экстракорпорального оплодотворения. Цитология. 2017; 59(7): 462-6.
- Иб/агимова Э.О., Долгушина Н.В., Сы/кашева А.Г., Романов А.Ю., Языкова О.И., Мака/ова Н.П. Роль вспомогательного хетчинга в программах лечения бесплодия методами вспомогательных репродуктивных технологий: обзор литературы. Гинекология. 2016; 18(2): 44-7.
- Долгушина Н.В., Иб/агимова Э.О., Романов А.Ю., Мака/ова Н.П., Довгань А.А., Сы/кашева А.Г., Калинина Е.А. Роль проназного хетчинга в повышении эффективности программ вспомогательных репродуктивных технологий. Акушерство и гинекология. 2018; (3): 70-5. https://dx.doi.org/10.18565/aig.2018.3.70-74
- Ковальская Е.В., Сы/кашева А.Г., Романов А.Ю., Мака/ова Н.П., Долгушина Н.В. Современные представления о компактизации эмбрионов человека в условиях in vitro. Технологии живых систем. 2017; (1): 25-35.
- Biggers J.D., Summers M.C. Choosing a culture medium: making informed choices. Fertil Steril. 2008; 90(3): 473-83 doi: 10.1016/j.fertnstert.2008.08.010
- Loutradis D., Drakakis P., KallianidisK., Sofikitis N., Kallipolitis G., Milingos S., et al. Biological factors in culture media affecting in vitro fertilization, preimplantation embryo development, and implantation. Ann N Y Acad Sci. 2000; 900: 325-35. doi: 10.1111/j.1749-6632.2000.tb06245.x
- Chronopoulou E., Harper J.C. IVF culture media: past, present and future. Hum Reprod Update. 2015; 21(1): 39-55. doi: 10.1093/humupd/dmu040
- Brison D.R., Houghton F.D., Falconer D., Roberts S.A., Hawkhead J., Humpherson P.G., et al. Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover. Hum Reprod. 2004; 19(10): 2319-24. doi: 10.1093/humrep/deh409
- Thompson J.G. Culture without the petridish. Theriogenology. 2007; 67(1):16- 20. doi: 10.1016/j.theriogenology.2006.09.016
- Gardner D.K., Lane M. Ex vivo early embryo development and effects on gene expression and imprinting. Reprod Fertil Dev. 2005; 17(3): 361-70. doi: 10.1071/rd04103
- Isachenko V., Maettner R., Sterzik K., Strehler E., Kreinberg R., Hancke K., et al. In-vitro culture of human embryos with mechanical micro-vibration increases implantation rates. Reprod Biomed Online. 2011; 22(6): 536-44. doi: 10.1016/j. rbmo.2011.02.006
- Muglia U., Motta P.M. A new morpho-functional classification of the Fallopian tube based on its three-dimensional myoarchitecture. Histol Histopathol. 2001; 16(1): 227-37. doi: 10.14670/HH-16.227
- Lyons R.A., Djahanbakhch O., Mahmood T., Saridogan E., Sattar S., SheaffM.T., et al. Fallopian tube ciliary beat frequency in relation to the stage of menstrual cycle and anatomical site. Hum Reprod. 2002; 17(3): 584-8. doi: 10.1093/ humrep/17.3.584
- Lyons R.A., Saridogan E., Djahanbakhch O. The reproductive significance of human Fallopian tube cilia. Hum Reprod Update. 2006; 12(4): 363-72. doi: 10.1093/humupd/dml012
- Isachenko E., Maettner R., Isachenko V., Roth S., Kreienberg R., Sterzik K. Mechanical agitation during the in vitro culture of human pre-implantation embryos drastically increases the pregnancy rate. Clin Lab. 2010; 56(11- 12):569-76. PMID: 21141442
- Xie Y., Wang F., Zhong W., Puscheck E., Shen H., Rappolee D.A. Shear stress induces preimplantation embryo death that is delayed by the zona pellucida and associated with stress-activated protein kinase-mediated apoptosis. Biol Reprod. 2006; 75(1): 45-55. doi: 10.1095/biolreprod.105.049791
- Matsuura K., Hayashi N., Kuroda Y., Takiue C., Hirata R., Takenami M., et al. Improved development of mouse and human embryos using a tilting embryo culture system. Reprod Biomed Online. 2010; 20(3): 358-64. doi: 10.1016/j. rbmo.2009.12.002
- Fauci L.J., Dillon R. Biofluidmechanics of reproduction. Annu Rev Fluid Mech. 2006; 38(1): 371-94. doi: 10.1146/annurev.fluid.37.061903.175725
- Foo J.Y.A., Lim C.S. Biofluid mechanics of the human reproductive process: modelling of the complex interaction and pathway to the oocytes. Zygote. 2008; 16(4): 343-54. doi: 10.1017/S0967199408004899
- Romanov A.Y., Silachev D.N., Makarova N.P., Dolgushina N. V. Effect of Mechanical Microvibration on the Quality of Human Embryos during In Vitro Culturing and Outcomes of Assisted Reproduction Technologies. Bull Exp Biol Med. 2018; 165(4): 544-7. ID: mdl-30121919
- Романов А.Ю., Силачев Д.Н., Мака/ова Н.П., Долгушина Н.В. Влияние механической микровибрации на качество эмбрионов человека при культивировании in vitro и исходы программ вспомогательных репродуктивных технологий. Клеточные технологии в биологии и медицине. 2018; (2): 86-90.
- Приказ Минздрава России от 30.08.2012 N 107н (ред. от 11.06.2015) «О порядке использования вспомогательных репродуктивных технологий, противопоказаниях и ограничениях к их применению»
- Hur Y.S., Ryu E.K., Yoon S.H., Lim K.S., Lee W.D., Lim J.H. Comparison of static culture, micro-vibration culture, and micro-vibration culture with coculture in poor ovarian responders. Clin Exp Reprod Med. 2016; 43(3): 146. doi: 10.5653/cerm.2016.43.3.146
- Isachenko V., Sterzik K., Maettner R., Isachenko E., Todorov P., Rahimi G., et al. In Vitro Microvibration Increases Implantation Rate After Embryonic Cell Transplantation. Cell Transplant. 2017; 26(5): 789-94. doi: 10.3727/096368916X693428
- Dobson S.J.A., Lao M.T., Michael E., Varghese A.C., Jayaprakasan K. Effect of transfer of a poor quality embryo along with a top quality embryo on the outcome during fresh and frozen in vitro fertilization cycles. Fertil Steril. 2018; 110(4): 655-60. doi: 10.1016/j.fertnstert. 2018.05.010
- Kwek L.K., Safari S.E., Tan H.H., Chan J.K., Nada S. Comparison between Single and Double Cleavage-Stage Embryo Transfers, Single and Double Blastocyst Transfers in a South East Asian In Vitro Fertilisation Centre. Ann Acad Med Singapore. 2018; 47(11): 451-4. PMID: 30578424
- Hur Y.S., Park J.H., Ryu E.K., Park S.J., Lee J.H., Lee S.H., et al. Effect of micro-vibration culture system on embryo development. J Assist Reprod Genet. 2013; 30(6):835-41. doi: 10.1007/s10815-013-0007-0
Arquivos suplementares
![](/img/style/loading.gif)