Characteristics of the embryological stage of infertility treatment using assisted reproductive technologies depending on the total antioxidant capacity of native ejaculate


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Objective: Ta investigate the impact af ejaculate total antiaxidant capacity af the ejaculate an fertilization and embrya develapment in cauples with different types af infertility and determine the value af the tatal antiaxidant capacity as a biomarker far predicting the outcomes af assisted reproductive technology (ART). Materials and methods: The study included 50 infertile cauples wha underwent in vitra fertilizatian using intracytaplasmic sperm injection (ICSI). On the day af transvaginalpunctures, native ejaculate was collected from their spouses ta test far total antiaxidant capacity using the FORM 3000 device using the FORD kit. Results: Embryos obtained with sperm from the ejaculate with higher total antiaxidant capacity reached the late blastocyst stage an day 5. Embryos obtained with sperm from the ejaculate with lower total antiaxidant capacity by day 5 developed ta morulae ar early blastocyst stage (p=0.03). There was a negative correlation between the total antiaxidant capacity af the ejaculate and the percentage af fertilized oocytes. No correlation was found between total antiaxidant capacity and ART outcomes in couples with different types af infertility. Conclusion: Total ejaculate antiaxidant capacity af native ejaculate before in vitro fertilization affects the characteristics af the embryalagical stage af ART. A high level af oxidative stress was significantly associated with a decrease in the fertilization rate. At the same time, the pregnancy rate was not associated with the level af oxidative stress in male germ cells. Careful pre-pregnancy care and conscious parenting are necessary ta improve the effectiveness af ART in men with high levels af oxidative stress in the ejaculate through an increase in the number af zygates.

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Sobre autores

Diana Agadzhanyan

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

MD, Postgraduate Student, BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Natalia Lobanova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: n_lobanova@oparina4.ru
Junior Researcher, BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

Veronika Smolnikova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_smolnikova@oparina4.ru
Dr. Med. Sci., Associate Professor, Leading Researcher, Department of IVF named after Professor BV. Leonov

Natalya Makarova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: np_makarova@oparina4.ru
Dr. Bio. Sci., Leading Researcher, Department of IVF named after Professor BV. Leonov

Aleksey Krasnyi

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: alexred@list.ru
PhD (Bio), Head of the Laboratory of Cytogy

Valeriya Shchipitsyna

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Junior Researcher at the Laboratory of Cytogy

Alsu Sadekova

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: sialsad@gmail.com
PhD (Bio), Researcher at the Laboratory of Cytogy

Diana Kokoeva

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: d_kokoeva@oparina4.ru
PhD, Junior Researcher at the Laboratory of Cytogy

Elena Kalinina

Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_kalinina@oparina4.ru
Dr. Med. Sci., Professor, Head of the BV. Leonov Department of Assisted Technologies for the Treatment of Infertility

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