Diagnosis of squamous intraepithelial lesions and cervical cancer based on the quantitative assessment of microRNA expression


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Abstract

Objective. To estimate the possibility of differential diagnosis of the severity of cervical squamous intraepithélial lesions on the basis of quantitative assessment of microRNA expression: has-mir-22-3p, has-mir-25*-3p, and has-mir-92a-5p in cervical epithelial scrapings. Subjects and methods. The investigation enrolled 95 women aged 18 to 49 years (mean age, 32.7±0.5 years) who were divided into 4 groups: 1) 32 patients without cervical disease; 2) 31 patients with low-grade squamous intraepithelial lesion; 3) 26 patients with high-grade squamous intraepithelial lesion; 4) 6 patients with cervical cancer. For a clinical diagnosis, cytological and histological examinations, extended colposcopy, and HPV testing were used and the level of microRNA expression was measured by quantitative real-time PCR. Results. In cervical squamous intraepithelial lesions, the synthesis of mir-22 was considerably decreased and the expression of mir-92a was, on the contrary, increased. The expression of mir-25 was elevated Insignificantly. A logistic regression analysis could reveal the mir-22 and mir-92a expression thresholds that allowed the differentiation of cervical cancer with a diagnostic accuracy of more than 80% in both patients without visible abnormalities and those with squamous intraepithelial lesions. Conclusion. Along with traditional methods, the investigation permits the expression level of miRNAs, mir-22, and mir-92a to be considered as an informative molecular marker to predict the extent of involvement of the cervical epithelium in pre-cancer and cancer of the cervix uteri.

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About the authors

Viktoria Fedorovna Chernova

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

PhD student

Vitaly Nikolaevich Karnaukhov

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_karnauhov@oparina4.ru
Junior Researcher, Laboratory of Molecular Genetic Methods

Leonid Zakievich Faizullin

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: l_faizullin@oparina4.ru
Cand. Sc. (Biology), Leading Researcher, Laboratory of Molecular Genetic Methods

Guldana Raufovna Bairamova

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: g_bairamova@oparina4.ru
doctor of medical sciences, head of the clinical work of the scientific and polyclinic department

Evgenia Aleksandrovna Kogan

I.M. Sechenov First Moscow State Medical University

Email: koganevg@gmail.com
MD, Professor, Head of the Department of Pathological Anatomy

Guranda Merabovna Mzarelua

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: mzareluag@mail.ru
graduate student

Niso Mirzoevna Nazarova

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: grab2@yandex.ru
MD, PhD, Senior Researcher

Andrey Vladimirovich Kozachenko

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_kozachenko@oparina4.ru
leading research assistant of the gynecological department

Dmitriy Yur'evich Trofimov

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: d_trofimov@oparina4.ru
Doctor of Biological Sciences, Head of the Laboratory of Molecular Genetic Methods

Vera Nikolaevna Prilepskaya

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: VPrilepskaya@mail.ru
MD, PhD, Professor, Deputy Director for Science

Gennady Tikhonovich Sukhikh

Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: g_sukhikh@oparina4.ru
Doctor of Medical Sciences, Academician of the Russian Academy of Sciences, Director

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