COMPARATIVE ANALYSIS OF THE USE OF MASS SPECTROMETRY AND AUTOMATED FLOW CYTOMETRY TO SCREEN FOR BACTERIURIA


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Objective. To determine the efficiency of current bacteriuria diagnostic techniques (mass spectrometry, flow cytofluorometry) by conducting a parallel comparative study and choosing the most optimal diagnostic procedure for urinary tract infections (URI). Subject and methods. A comprehensive analysis of 790 urine samples was made using three methods: fluorescence flow cytofluorometry, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and culture. Results. The three methods yielded identical results in 65.6% and discrepancies in 34.4%. Conclusion. Urine culture remains the gold standard in the diagnosis of URI. In true bacteriuria, MALDI-TOF mass spectrometry can directly identify promptly urinary microorganisms in high titers, reducing the time to start etiotropic therapy. Flow cytofluorometric urinalysis can detect bacteriuria at a concentration of >10 5 CFU/ml or its absence with a negative leukocyte test.

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作者简介

T. PRIPUTNEVICH

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

S. ZAITSEVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

M. ZAVYALOVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

A. MELKUMYAN

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

T. TETERINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

E. ILYINA

Research Institute of Physicochemical Medicine, Federal Biomedical Agency of Russia

S. MITROKHIN

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

I. APOLIKHINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

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