COMPARATIVE ANALYSIS OF THE USE OF MASS SPECTROMETRY AND AUTOMATED FLOW CYTOMETRY TO SCREEN FOR BACTERIURIA


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Abstract

Objective. To determine the efficiency of current bacteriuria diagnostic techniques (mass spectrometry, flow cytofluorometry) by conducting a parallel comparative study and choosing the most optimal diagnostic procedure for urinary tract infections (URI). Subject and methods. A comprehensive analysis of 790 urine samples was made using three methods: fluorescence flow cytofluorometry, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and culture. Results. The three methods yielded identical results in 65.6% and discrepancies in 34.4%. Conclusion. Urine culture remains the gold standard in the diagnosis of URI. In true bacteriuria, MALDI-TOF mass spectrometry can directly identify promptly urinary microorganisms in high titers, reducing the time to start etiotropic therapy. Flow cytofluorometric urinalysis can detect bacteriuria at a concentration of >10 5 CFU/ml or its absence with a negative leukocyte test.

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About the authors

T. V PRIPUTNEVICH

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

S. A ZAITSEVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

M. G ZAVYALOVA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

A. R MELKUMYAN

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

T. A TETERINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

E. N ILYINA

Research Institute of Physicochemical Medicine, Federal Biomedical Agency of Russia

S. D MITROKHIN

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

I. A APOLIKHINA

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

References

  1. Набер К.Г., Бергман Б., Бишоп М.К., Бьерклунд-Йохансен Т.Е., Ботто Г., Лобель Б. и др. Рекомендации Европейской ассоциации урологов по лечению инфекций мочевыводящих путей и инфекций репродуктивной системы у мужчин. Клиническая микробиология и антимикробная химиотерапия. 2002; 4(4): 347-63.
  2. Kouri T., Fogazzi G., Gant V., Hallander H., Hofmann W., Guder W., eds. ECLM. European urinalysis guidelines. Scand. J. Clin. Lab. Invest. 2000; 60(Suppl. 231): 1-86.
  3. Научный отчет о результатах многоцентрового исследования динамики антибиотикорезистентности возбудителей инфекций мочевых путей в различных субпопуляциях пациентов «ДАРМИС». Смоленск; 2011.
  4. Франц М., Хорл У. Наиболее частые ошибки диагностики и ведения инфекции мочевых путей (ИМП). Нефрология и диализ. 2002; 2(4): 340-7.
  5. Hanno P.M., Wein A.J., Malkowicz S.B. Penn clinical manual of urology. 1st ed. Philadelphia: Saunders Elsevier; 2007: 155-75.
  6. Hooton T.M., Stamm W.E. Diagnosis and treatment of uncomplicated urinary tract infection. Infect. Dis. Clin. North Am. 1997; 11(3): 551-81.
  7. Abrams P., Cardozo L., Khoury S., Wein A., eds. Incontinence: Third International Consultation. Paris: Health Publication LTD; 2005; vol.1: 21.
  8. National Institute for Health and Clinical Excellence. Urinary incontinence: the management of urinary incontinence in women. NICE clinical guideline 40. October 2006.
  9. Kunin C.M., White L.V., Hua T.H. A reassessment of the importance of “low-count” bacteriuria in young women with acute urinary symptoms. Ann. Intern. Med. 1993; 119(6): 454-60.
  10. Hessdoerfer E., Jundt K., Peschers U. Is a dipstick test sufficient to exclude urinary tract infection in women with overactive bladder. Int. Urogynecol. J. 2011; 22(2): 229-32.
  11. Ferreira L., Sánchez-Juanes F., González-Avila M., Cembrero-Fuciños D., Herrero-Hernández A., González-Buitrago J.M., Muñoz-Bellido J.L. Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 2010; 48(6): 2110-5.
  12. Schwarz G., Maier T., Kostrzewa M., Boogen C., Weller U. Rapid identification of bacteria causing urinary tract infections by MALDI-TOF. In: Abstract of the 48th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) and the 46th Infectious Diseases Society of America (IDSA) Washington, DC, October 25-26, 2008: abstr. D-4025.
  13. Wang J., Zhang Y., Xu D., Shao W., Lu Y. Evaluation of the Sysmex UF-1000i for the diagnosis of urinary tract infection. Am. J. Clin. Pathol. 2010; 133(4): 577-82.
  14. Broeren M.A., Bahçeci S., Vader H.L., Arents N.L. Screening for urinary tract infection with the Sysmex UF-1000i urine flow cytometer. J. Clin. Microbiol. 2011; 49(3): 1025-9.
  15. Borovskaya A., Ilina E., Govorun V., Morozova O., Ditmarova J., Kruglov A. et al. Bacterial species identification directly from urine samples by MALDI-TOF MS fingerprinting. In: 19-th European congress of clinical microbiology and infectious diseases. Helsinki, Finland 16-19 May 2009: abstr. P1065.
  16. Isenberg H.D., ed. Clinical microbiology procedures handbook. Washington, DC: American Society of Microbiology; 1992: 1.17.6 - 1.17.7.
  17. Маянский Н.А., Мотузова О.В., Калакуцкая А.Н., Семикина Е.Л., Катосова Л.К. Скрининг бактериурии у детей с помошью автоматизированной проточной цитометрии на анализаторе Sysmex UF-1000i. Вопросы диагностики в педиатрии. 2011; 3(3): 12-6.
  18. Hrabák J., Chudácková E., Walková R. Matrix-assisted laser desorption ionization - time of flight (MALDI-TOF) mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis. Clin. Microbiol. Rev. 2013; 26(1): 103-4.

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