Estimation of the importance of endometrial molecular genetic markers in predicting the outcome of in vitro fertilization pregnancy


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Objective. To study the mRNA expression profile of the genes involved in the processes of implantation in the endometrium in the cycle preceding in vitro fertilization (IVF) treatment. Subjects and methods. A total of 121 patients aged 24 to 42 years with tuboperitoneal factor infertility were examined. According to the results of a morphological study of the women’s endometrium were divided into two groups: 1) 55 patients in whom the endometrium corresponded to an early secretory phase; 2) 62 patients, in whom it corresponded to a mid-secretory phase; 4 women were diagnosed as having a late proliferative stage and excluded because of they constituted a minor group. Endometrial biopsy specimens were morphologically examined during the implantation window period in the cycle preceding superovulation. The endometrial mRNA expression of 36 genes was analyzed by real-time reverse transcription and qualitative polymerase chain reaction assays. Results. Significant differences were found in the expression of MMP-7, VEGF-A, IL-1b, IL-2, IL-8, IL-18, TNF-α, IL-10, and TGF-b mRNA in the early secretory phase and in that of IGFBP2 mRNA in the mid-secretory phase in patients with biochemical and clinical pregnancy versus those with a negative IVF outcome. A procedure was developed to predict the occurrence of pregnancy on the basis of an integral estimation of IL-15, MMP-11, and VEGF-A mRNA.

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作者简介

M. Maslova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: mayay20l2@mail.ru
postgraduate student, Department of assisted reproductive technologies

V. Smolnikova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: centr.eko@mail.ru
MD, PhD, senior research fellow, Department of assisted reproductive technologies

A. Donnikov

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
Ph.D. scientist of the laboratory of molecular genetics methods

O. Burmenskaya

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: bourmenska@mail.ru
Ph.D. scientist of the laboratory of molecular genetics methods

T. Demura

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: demura-t@yandex.ru
Senior Researcher, Ph.D, 1st Pathology Department

V. Tabolova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tabol93@rambler.ru
graduate students, Department of Fertility Preservation and Restoration

I. Korneeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: irina.korneeva@inbox.ru
MD, PhD, senior research fellow, Department of Fertility Preservation and Restoration

参考

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