Estimation of the importance of endometrial molecular genetic markers in predicting the outcome of in vitro fertilization pregnancy


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Abstract

Objective. To study the mRNA expression profile of the genes involved in the processes of implantation in the endometrium in the cycle preceding in vitro fertilization (IVF) treatment. Subjects and methods. A total of 121 patients aged 24 to 42 years with tuboperitoneal factor infertility were examined. According to the results of a morphological study of the women’s endometrium were divided into two groups: 1) 55 patients in whom the endometrium corresponded to an early secretory phase; 2) 62 patients, in whom it corresponded to a mid-secretory phase; 4 women were diagnosed as having a late proliferative stage and excluded because of they constituted a minor group. Endometrial biopsy specimens were morphologically examined during the implantation window period in the cycle preceding superovulation. The endometrial mRNA expression of 36 genes was analyzed by real-time reverse transcription and qualitative polymerase chain reaction assays. Results. Significant differences were found in the expression of MMP-7, VEGF-A, IL-1b, IL-2, IL-8, IL-18, TNF-α, IL-10, and TGF-b mRNA in the early secretory phase and in that of IGFBP2 mRNA in the mid-secretory phase in patients with biochemical and clinical pregnancy versus those with a negative IVF outcome. A procedure was developed to predict the occurrence of pregnancy on the basis of an integral estimation of IL-15, MMP-11, and VEGF-A mRNA.

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About the authors

M. A Maslova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: mayay20l2@mail.ru
postgraduate student, Department of assisted reproductive technologies

V. Yu Smolnikova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: centr.eko@mail.ru
MD, PhD, senior research fellow, Department of assisted reproductive technologies

A. E Donnikov

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: a_donnikov@oparina4.ru
Ph.D. scientist of the laboratory of molecular genetics methods

O. V Burmenskaya

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: bourmenska@mail.ru
Ph.D. scientist of the laboratory of molecular genetics methods

T. A Demura

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: demura-t@yandex.ru
Senior Researcher, Ph.D, 1st Pathology Department

V. K Tabolova

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: tabol93@rambler.ru
graduate students, Department of Fertility Preservation and Restoration

I. E Korneeva

Academician V.I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia

Email: irina.korneeva@inbox.ru
MD, PhD, senior research fellow, Department of Fertility Preservation and Restoration

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