The results of the examination of cervical-vaginal microbiota in pregnant women with threatened preterm birth using a real-time polymerase chain reaction


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Objective.To investigate the prevalence of urogenital infections and status of the cervical-vaginal microbiota in pregnant women with threatened preterm birth using real-time polymerase chain reaction (PCR). Material and methods. We conducted a cross-sectional study of300pregnant women at 27 to 32 weeks’ gestation, of which 118 were diagnosed with threatened preterm labor, and 182 had a late uncomplicated pregnancy (control group). We analyzed the results of antenatal screening for urogenital infections, which included real-time PCR detection of etiologically significant pathogens associated with bacterial vaginosis, vulvovaginal candidiasis, aerobic vaginitis, and the causative agents of four sexually transmitted infections (Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis). All study participants attended antenatal clinics. Results Among pregnant women with threatened preterm birth and women in the control group, the prevalence of mixed infections, bacterial vaginosis, aerobic vaginitis, vulvovaginal candidiasis, and infection caused by Mycoplasma genitalium was 14% and 4% (p <0.01), 17% and 7% (p <0.05), 12% and 3% (p < 0.05), 16% and 9% (p = 0.08), and 2% and 1% (p > 0.05), respectively. No women in the control group had chlamydial infection and gonorrhea, while they were found in 3% (p < 0.05) and 0.8% (n = 1) (p < 0.05) among patients with threatened preterm labor, respectively. The vaginal concentration of Lactobacillus spp. was significantly higher in the women in the control group compared with the patients with threatened preterm birth (8.24 ± 0.67 and 7.38 ± 1.27GE/ml;p <0.001). Conclusion Real-time PCR screening for female genital tract infections revealed significantly higher rates of mixed infections, bacterial vaginosis, aerobic vaginitis, chlamydial infection among pregnant women with threatened preterm birth.

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作者简介

Yuliya Dobrokhotova

N.I. Pirogov Russian National Research Medical University

Email: pr.dobrohotova@mail.ru
SPIN 代码: 2925-9948
Dr.Med.Sci., Professor, Head of the Department of Obstetrics and Gynecology, Faculty of Medicine

Karina Bondarenko

N.I. Pirogov Russian National Research Medical University

Email: karinabond@mail.ru
SPIN 代码: 9976-0735
Ph.D., Teaching Assistant at the Department of Obstetrics and Gynecology, Faculty of Medicine

Aleksandr Gushchin

Central Research Institute of Epidemiology of Rospotrebnadzor

Email: aguschin1965@pcr.ru
SPIN 代码: 3496-6893
Ph.D. (bio.sci.), Head of the Laboratory for Molecular Diagnostics and Epidemiology of Infections of Reproductive Organs

Tat’yana Rumyantseva

Central Research Institute of Epidemiology of Rospotrebnadzor

Email: Ivanovatatiana86@yandex.ru
SPIN 代码: 4037-5114
Researcher at the Laboratory of Molecular Diagnostics and Epidemiology of Infections of Reproductive Organs, Obstetrician-gynecologist at the Clinical Diagnostics and Research Center

T. Dolgova

N.I. Pirogov Russian National Research Medical University

Pavel Kuznetsov

N.I. Pirogov Russian National Research Medical University

Email: poohsmith@mail.ru
SPIN 代码: 6683-4300
Ph.D., Associate Professor at the Department of Obstetrics and Gynecology, Faculty of Medicine

Lela Dzhokhadze

Central Research Institute of Epidemiology of Rospotrebnadzor

Email: anton-b1@mail.ru
SPIN 代码: 3482-8630
Ph.D., Teaching Assistant at the Department of Obstetrics and Gynecology, Faculty of Medicine

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