Possibilities for predicting recurrent uterine myoma by plasma lipidomic analysis


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Objective. To predict recurrent uterine fibroids (UFs) by direct mass spectrometry. Subjects and methods. A plasma lipidomic analysis was carried out in 81 patients treated at the V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, including 35 patients with newly diagnosed UFs, 31 patients diagnosed with recurrent UFs (RUFs), and 15 comparison group patients who underwent surgical treatment for infertility, intrauterine septum. Semi-quantitation of blood lipid levels was performed by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). Mass spectrometric data were processed using the orthogonal projection to latent structure discriminant analysis (OPLS-DA) to build a statistical model for classifying samples and identifying potential biomarkers. The lipids were identified by their exact mass and characteristic tandem mass spectra (MS/MS). Results. The presented patient groups differed in the lipid composition of blood plasma by the presence and level of phospholipids, sphingomyelins, cholesterol esters, and triglycerides. The created statistical models made it possible to identify lipids, potential biomarkers of both the disease itself and its recurrences. Conclusion. This investigation confirms the implication of phospholipids and sphingolipids in the pathogenesis of UFs and opens up new possibilities for predicting RUFs.

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作者简介

N. Tonoyan

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: tonnar.l3@bk.ru

A. Tokareva

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: alisa.tokareva@phystech.edu

V. Chagovets

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: vvchagovets@gmail.com

N. Starodubtseva

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: n_starodubtseva@oparina4.ru

I. Kozachenko

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

L. Adamyan

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: adamyanleila@gmail.com

V. Frankevich

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation

Email: v_frankevich@oparina4.ru

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