Analysis of HLA class I gene (G) polymorphic alleles in pregnant women in the North-West Region of the Russian Federation
- 作者: Shengelia M.O.1, Bespalova O.N.1, Ivashchenko T.E.1, Frank N.I.1, Sultanov I.Y.1, Nasykhova Y.A.1, Glotov A.S.1
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隶属关系:
- D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
- 期: 编号 3 (2023)
- 页面: 99-108
- 栏目: Exchange of Experience
- URL: https://journals.eco-vector.com/0300-9092/article/view/323273
- DOI: https://doi.org/10.18565/aig.2022.270
- ID: 323273
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Background: The HLA class I system controls the interaction of all immunocompetent cells of the body and determines the recognition of its own and foreign (including altered own) cells, by triggering and realizing an immune response: during the implantation process, in the transplantation of foreign organs and tissues, in autoimmune diseases, and in the development of cancers. Thus, the data available in the world and Russian literature have demonstrated the ability of protein products of HLA-G gene expression to modulate the proliferation of immune system cells, as well as the strength and specificity of the immune response.
Objective: To conduct a molecular biological study of nine HLA-G gene polymorphic alleles in the group of pregnant women in the first trimester and in the population of the North-West Region of the Russian Federation and to identify the relationship to their family obstetric history.
Materials and methods. In 2019 to 2022, the D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology (Saint Petersburg) examined DNA samples from 180 dwellers in the North-West Region of the Russian Federation. Group 1 consisted of 33 pregnant women with reproductive disorders (one or more miscarriages, non-developing pregnancy, ART failures); Group 2 (control) included 29 pregnant women without their family obstetric history; a comparison group comprised 118 people from the population of the North-West Region of the Russian Federation, in which the investigators carried out an associative analysis of HLA-G gene polymorphic alleles (725*C/G, 3741*ins/del 14 bp) and HLA-G*01 alleles (01, G*01:02, G*01:03, G*01:04, G*01:05N, G*01:06 and G*01:07).
Results: The analysis of 3741*ins/del 14 bp polymorphism showed that the Del/Del genotype frequency was 13/33 (39.40%) in pregnant women with a history of reproductive losses, 5/29 (17.24%) in the control group; p = 0.052), and the allele Ins was detected 1,4 times more often in the group without a family obstetric history (20/33 (60.6%) and 24/29 (82.8%), respectively; p=0.056). According to the calculated odds ratio, the carriers of the combined Del/Del and C/C genotype were detected 9 times more frequently among patients with a history of reproductive losses compared to the controls (OR=8.96, CI 1.05;76.74). In Group 1, the HLA-G*01:05N allele was noted to be detected 3.5 times more often than in the population sample.
Conclusion: The findings may judge an association between HLA-G gene alleles and a family obstetric history and may be important in assessing the risk of early reproductive losses and in developing new prevention and treatment strategies for this pregnancy complication.
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作者简介
Margarita Shengelia
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
编辑信件的主要联系方式.
Email: bakleicheva@gmail.com
ORCID iD: 0000-0002-0103-8583
Junior Researcher
俄罗斯联邦, Saint PetersburgOlesya Bespalova
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: shiggerra@mail.ru
ORCID iD: 0000-0002-6542-5953
Dr. Med Sci., Deputy Director for Research
俄罗斯联邦, Saint PetersburgTatyana Ivashchenko
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: tivashchenko2011@mail.ru
ORCID iD: 0000-0002-8549-6505
Dr. Dio. Sci., Professor, Leading Researcher at the Medical Genetic Center (Department of Genomic Medicine)
俄罗斯联邦, Saint PetersburgNadezhda Frank
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: ncotta@mail.ru
nurse at the Medical Genetic Center
俄罗斯联邦, Saint PetersburgIskender Sultanov
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: bakleicheva@gmail.com
Laboratory Assistant-Researcher at the Laboratory of Genomics with a Group of Bioresource Collections
俄罗斯联邦, Saint PetersburgYulia Nasykhova
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: yulnasa@gmail.com
ORCID iD: 0000-0002-3543-4963
Ph.D. (Bio), Head of the Genomics with a Group of Bioresource Collections
俄罗斯联邦, Saint PetersburgAndrey Glotov
D.O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology
Email: anglotov@mail.ru
ORCID iD: 0000-0002-7465-4504
Dr. Bio. Sci., Head of the Medical Genetic Center
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