Phenotypic profile of peripheral blood mononuclear cells in preeclampsia

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Objective: To investigate the phenotypic profile of mononuclear cells in the peripheral blood of pregnant women with preeclampsia.

Materials and methods: This study included 38 pregnant women. The study group included 20 women (10 with mild preeclampsia and 10 with severe preeclampsia). The control group comprised 18 women with healthy pregnancies. Flow cytometry was used to determine the expression by monocytes and lymphocytes of costimulatory inflammatory factors CD40, CD80, CD86, surface signaling molecules of the "don't eat me" pathway CD24 and CD47, surface costimulatory receptors CD28 and CD152, and Fc receptor CD16.

Results: Lymphocytes in the blood of pregnant women in the study group had increased expression of CD28 and CD16, monocytes had increased expression of CD152 and CD86, and there was a higher content of monocytes expressing CD16 in this group. Correlation analysis showed a relationship between the level of expression of CD152 and CD86 in monocytes and the content of monocytes expressing CD16 in women in both groups.

Conclusion: The relationship between the expression levels of CD16, CD152, and CD86 indicates the possibility of their involvement in the same CD86-mediated signaling pathway leading to the activation of the CD152 receptor, followed by the expression of the CD16 Fc receptor. These findings suggest that these factors may be potential markers for PE.

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作者简介

Aleksey Krasnyi

Academician V.I. Kulakov National Medical Research Centre of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

编辑信件的主要联系方式.
Email: alexred@list.ru
ORCID iD: 0000-0001-7883-2702

PhD, Head of the Cytology Laboratory

俄罗斯联邦, Moscow

Natalia Kan

Academician V.I. Kulakov National Medical Research Centre of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: kan-med@mail.ru
ORCID iD: 0000-0001-5087-5946
SPIN 代码: 5378-8437
Scopus 作者 ID: 57008835600
Researcher ID: B-2370-2015

MD, PhD, Deputy Director of Science

俄罗斯联邦, Moscow

Dzhamilia Mirzabekova

Academician V.I. Kulakov National Medical Research Centre of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: Jamilya1705@yandex.ru
ORCID iD: 0000-0002-2391-3334

PhD student

俄罗斯联邦, Moscow

Victor Tyutyunnik

Academician V.I. Kulakov National Medical Research Centre of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: tioutiounnik@mail.ru
ORCID iD: 0000-0002-5830-5099
SPIN 代码: 1963-1359
Scopus 作者 ID: 56190621500
Researcher ID: B-2364-2015

Professor, MD, PhD, Leading Researcher at the Research and Development Service

俄罗斯联邦, Moscow

Ekaterina Panasenko

Academician V.I. Kulakov National Medical Research Centre of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: e_panasenko@oparina4.ru

Researcher at the Cytology Laboratory

俄罗斯联邦, Moscow

Alsu Sadekova

Academician V.I. Kulakov National Medical Research Centre of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: a_sadekova@oparina4.ru
ORCID iD: 0000-0003-4726-7477

PhD, Researcher at the Cytology Laboratory

俄罗斯联邦, Moscow

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2. Fig. 1. Expression level of CD28 by lymphocytes, CD152 and CD86 by monocytes

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3. Fig. 2. Spearman correlation coefficient in the main group (A) and comparison group (B). *p<0.05; **p<0.001

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