Application of fluorescent in situ hybridization in the diagnosis of bacterial vaginosis
- 作者: Savicheva A.M.1,2,3, Krysanova A.A.1,2,3, Shalepo K.V.1,2,3, Spasibova E.V.1,2,3, Budilovskaya O.V.1,2,3, Khusnutdinova T.A.1,2,3, Tapilskaya N.I.1,2, Kogan I.Y.1,4, Swidsinski A.V.5,3,6, Swidsinski S.5,3
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隶属关系:
- D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction
- St. Petersburg State Pediatric Medical University, Ministry of Health of Russia
- International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
- St. Petersburg State University
- Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charit, Hospital
- I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia
- 期: 编号 12 (2023)
- 页面: 68-77
- 栏目: Reviews
- URL: https://journals.eco-vector.com/0300-9092/article/view/625888
- DOI: https://doi.org/10.18565/aig.2023.129
- ID: 625888
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详细
Bacterial vaginosis (BV) is a polymicrobial biofilm vaginal syndrome characterized by a high prevalence, recurrence rate and associated complications, including preterm birth, infertility and a higher risk of sexually transmitted infections. Traditional diagnostic methods used to detect the disease do not provide complete information on the morphology, number, and spatial arrangement of microorganisms associated with BV. Fluorescence in situ hybridization (FISH) combines the accuracy of molecular genetics with the informative power of microscopy to visualize the relationships between bacteria in their natural microhabitat, such as the biofilm in BV. The persistence of biofilm composed of BV-associated microorganisms is one of the most likely pathways for recurrence and is an important diagnostic marker for the disease. The review of the literature presents the history of the use of FISH, outlines its basic principles and demonstrates its advantages in the diagnosis of bacterial vaginosis, especially its recurrent forms.
Conclusion: The use of the FISH method may not only change the understanding of BV pathogenesis, but it can also identify the etiological agent in each particular case, diagnose biofilm/non-biofilm vaginosis by determining the spatial relationship of bacteria to each other and to epithelial cells, predict the recurrence of the disease and choose the appropriate therapy.
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作者简介
Alevtina Savicheva
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
Email: savitcheva@mail.ru
ORCID iD: 0000-0003-3870-5930
Dr. Med. Sci., Professor, Head of the Department of Medical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Head of the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; Head of the International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
俄罗斯联邦, St. Petersburg; St. PetersburgAnna Krysanova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
编辑信件的主要联系方式.
Email: krusanova.anna@mail.ru
ORCID iD: 0000-0003-4798-1881
PhD, MD, Researcher, Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Assistant, Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; PhD, MD, Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
俄罗斯联邦, St. Petersburg; St. PetersburgKira Shalepo
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
Email: 2474151@mail.ru
ORCID iD: 0000-0002-3002-3874
PhD, MD, Senior Researcher, Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Associate Professor, Department of Clinical Laboratory Diagnostics of AF and DPO, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; PhD, MD, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
俄罗斯联邦, St. Petersburg; St. PetersburgElena Spasibova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
Email: elena.graciosae@gmail.com
ORCID iD: 0009-0002-6070-4651
bacteriologist, Laboratory of Clinical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Assistant, Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; bacteriologist, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
俄罗斯联邦, St. Petersburg; St. PetersburgOlga Budilovskaya
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
Email: o.budilovskaya@gmail.com
ORCID iD: 0000-0001-7673-6274
PhD, MD, Senior Researcher, Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Assistant, Department of Clinical Laboratory Diagnostics of AF and DPO, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; PhD, MD, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
俄罗斯联邦, St. Petersburg; St. PetersburgTatiana Khusnutdinova
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
Email: husnutdinovat@yandex.ru
ORCID iD: 0000-0002-2742-2655
PhD, MD, Senior Researcher, Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Assistant, Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia; PhD, MD, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
俄罗斯联邦, St. Petersburg; St. PetersburgNatalya Tapilskaya
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State Pediatric Medical University, Ministry of Health of Russia
Email: tapnatalia@yandex.ru
ORCID iD: 0000-0001-5309-0087
Dr. Med. Sci., Professor, Leading Researcher of the Reproduction Department, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology; Professor, Department of Obstetrics and Ginecology, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia
俄罗斯联邦, St. Petersburg; St. PetersburgIgor Kogan
D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction; St. Petersburg State University
Email: ovr@ott.ru
ORCID iD: 0000-0002-7351-6900
Corresponding Member of the RAS, Dr. Med. Sci., Professor, Director, D.O. Ott Reasearch Institute of Obstetrics, Gynecology and Reproductology; Professor, St. Petersburg State University
俄罗斯联邦, St. Petersburg; St. PetersburgAlexander Swidsinski
Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charit, Hospital; International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms; I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia
Email: alexander.swidsinski@charite.de
ORCID iD: 0000-0002-7071-0417
Head of the Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charité CCM, Medizinische Klinik, Universitätsmedizin; Co-Head of the International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
德国, Berlin; MoscowSonia Swidsinski
Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charit, Hospital; International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
Email: alexander.swidsinski@charite.de
bacteriologist of the Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charité CCM, Medizinische Klinik, Universitätsmedizin; bacteriologist of the International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms
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