Expression patterns of maternal and fetal tissue and exosomal microRNAs during pre-induction of labor (pilot study)

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Successful preparation of the female body for childbirth, carried out through various mechanisms of labor initiation and activities aimed at early delivery, is necessary to reduce obstetric and neonatal complications. These mechanisms, which combine the inflammatory response in the myometrium, fetal signaling, and physiological aging of membranes, are based on processes and signaling pathways. Coordinated regulation of these processes is performed using microRNAs (miRNAs).

Objective: This study aimed to investigate the expression of maternal and fetal tissues and exosomal miRNAs as signaling molecules for the initiation of labor during cervical ripening.

Materials and methods: This pilot study assessed maternal and fetal tissue and exosomal miRNA expression using quantitative real-time reverse transcription-PCR in 22 pregnant women divided into two cohorts: «labor without pre-induction» (cohort I, n=10) and «pre-induction of labor» (cohort II, n=12). Cohort I was further divided into two groups: pregnant women with spontaneous labor (group Ia, n=5) and pregnant women who underwent a planned cesarean section before the onset of labor (group Ib, n=5). Cohort II included pregnant women with a positive response to pre-induction of labor (group IIa, n=5) and pregnant women who did not achieve this effect (group IIb, n=7).

Results: The study found that the initial level of exosomal miR-181a-5p in the blood plasma before the pre-induction of labor was significantly different (p = 0.03) in pregnant women with a good response (cervical ripening and onset of labor) compared to those with no response. The level of exosomal miR-92a-3p in umbilical cord blood was higher than that in maternal blood before pre-induction (p=0.009). The expression of miR-454-3p and miR-548g-5p was detected only in umbilical cord blood samples, indicating their fetal origin. A pairwise comparison between the groups revealed a significantly higher level of let-7b-5p expression in the membranes in the successful pre-induction group than in the planned cesarean section group (p=0.02) and the group with no effect from pre-induction of labor (p=0.01). Similarly, in the myometrium, the expression of let-7b-5p was significantly higher in the successful pre-induction group than in the planned cesarean section group (p = 0.05).

Conclusion: The identified differences in the expression of tissue and exosomal miRNAs may be due to the highly coordinated regulation of different signaling pathways and the corresponding target genes involved in the mechanism of communication between the maternal and fetal compartments. This provides a basis for further research using larger cohorts of pregnant women.

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作者简介

Asiyat Gaidarova

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

编辑信件的主要联系方式.
Email: a_gaydarova@oparina4.ru
ORCID iD: 0000-0003-1415-3318

PhD Student

俄罗斯联邦, 117997, Moscow, Ac. Oparina str., 4

Vladislava Gusar

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_gusar@oparina4.ru
ORCID iD: 0000-0003-3990-6224

PhD, Senior Researcher at the Laboratory of Applied Transcriptomics of the Department of Systems Biology in Reproduction

俄罗斯联邦, 117997, Moscow, Ac. Oparina str., 4

Vitaliy Chagovets

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: v_chagovets@oparina4.ru
ORCID iD: 0000-0002-5120-376X

PhD, Head of the Laboratory of Metabolomics and Bioinformatics

俄罗斯联邦, 117997, Moscow, Ac. Oparina str., 4

Natalia Kan

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia

Email: kan-med@mail.ru
ORCID iD: 0000-0001-5087-5946

Professor, Dr.Med. Sci., Deputy Director for Science

俄罗斯联邦, 117997, Moscow, Ac. Oparina str., 4

Irina Edilberg

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Email: i_edilberg@oparina4.ru
ORCID iD: 0000-0003-4194-8730

PhD Student

俄罗斯联邦, 117997, Moscow, Ac. Oparina str., 4; 119991, Moscow, Trubetskaya str., 8-2

Oleg Baev

Academician V.I. Kulakov National Medical Research Centre for Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University)

Email: o_baev@oparina4.ru
ORCID iD: 0000-0001-8572-1971
SPIN 代码: 5378-8437
Scopus 作者 ID: 57008835600
Researcher ID: B-2370-2015

Dr. Med. Sci., Professor, Head of the 1st Maternity Department, Professor of the Department of Obstetrics, Gynecology, Perinatology and Reproductology

俄罗斯联邦, 117997, Moscow, Ac. Oparina str., 4; 119991, Moscow, Trubetskaya str., 8-2

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