Characteristics of CACNA1C protein expression levels in the round ligaments of the uterus in patients with genital prolapse
- 作者: Cheremin M.M.1, Smolnova T.Y.1, Krasnyi A.M.1, Sadekova A.A.1, Chuprynin V.D.1
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隶属关系:
- Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
- 期: 编号 7 (2024)
- 页面: 87-95
- 栏目: Original Articles
- URL: https://journals.eco-vector.com/0300-9092/article/view/635288
- DOI: https://doi.org/10.18565/aig.2024.147
- ID: 635288
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详细
Genital prolapse (GP) is a multifactorial disease that is influenced by various factors such as childbirth, obstetric trauma, age, and increased intra-abdominal pressure. However, not all individuals with these factors develop GP, highlighting the importance of gene and protein expression levels for understanding the underlying cause.
Objective: This study aimed to investigate the expression of the α1C subunit of the CaV1.2 calcium channel in the round ligaments of patients with GP.
Materials and methods: This study included 61 patients, with 31 in the GP group (group I, study group) and 30 with other gynecological conditions (group II, control group). The mean age of the patients was 55.6 and 46.5 years, respectively. In group I, 12/31 (38.7%) patients had grade II–III uterine and vaginal wall prolapse, whereas 15/31 (48.38%) and 4/31 (12.9%) patients had incomplete and complete GP, respectively. The control group did not have GP. The level of α1C protein expression in the round ligaments of the uterus was examined using Western blot.
Results: Group I exhibited lower α1C protein expression (3.034 [0.8108; 4.040] RU) than group II (4.098 [2.508; 6.543] RU) (p=0.0045). Reduced α1C protein expression in group I was associated with a sarcopenic phenotype, including muscle wasting and asthenia in 9/31 (29%), myopia in 7/31 (22%), hypotension in 16/31 (51%), constipation in 17/31 (54%), phlebopathy in 11/31 (35%), pelvic floor protrusion and relaxation in 16/31 (51%), apical GP in 24/31 (77%), hypermobility in 24/31 (77%), and flat feet in 15/31 (48%) patients.
Conclusion: Patients with GP had lower α1C protein expression than those in the control group. The low expression of α1C protein is indicative of a clinical symptom complex of connective tissue dysplasia (CTD), which aligns with the concept of a "sarcopenic phenotype." In patients with CTD and a sarcopenic phenotype, the labor process is associated with low α1C protein expression levels, which contributes to the development of GP. The specific form of GP (apical) is influenced by alterations in the calcium channel function, resulting in decreased α1C protein expression.
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作者简介
Mikhail Cheremin
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
编辑信件的主要联系方式.
Email: mkhrznt@gmail.com
ORCID iD: 0000-0002-8600-068X
Obstetrician-Gynecologist, PhD student
俄罗斯联邦, MoscowTatyana Smolnova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: smoltat@list.ru
ORCID iD: 0000-0003-3543-651X
Dr. Med. Sci., Senior Researcher at the Department of General Surgery
俄罗斯联邦, MoscowAlexey Krasnyi
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_krasnyi@oparina4.ru
ORCID iD: 0000-0001-7883-2702
PhD (Bio), Head of the Cytology Laboratory
俄罗斯联邦, MoscowAlsu Sadekova
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: a_sadekova@oparina4.ru
Researcher at the Cytology Laboratory
俄罗斯联邦, MoscowVladimir Chuprynin
Academician V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation
Email: v_chuprynin@oparina4.ru
ORCID iD: 0009-0003-7856-2863
PhD, Head of the Department of Surgery
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