DETERMINATION OF the TOXICITY CLASS OF A GRATIOLA OFFICINALIS L. PLANT EXTRACT, USING LARVAE CHIRONOMUS RIPARIUS AS A NEW TEST OBJECT

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Abstract

The herb golden pert (Gratiola Officinalis ) was used as starting material in the study. Magnitudes of the median lethal concentration (LC50) and accumulation character determined in larvae Chironomus riparius were taken as toxicity indicators on the organismal level. Results of the extract toxicity obtained with larvae were compared with those obtained with laboratory mice in terms of median lethal dose (LD50). A possibility of using Chironomid larvae Ch.riparius as a new test object in pre-clinical safety assessment of pharmacological substances was proved. A semi-lethal concentration (LC50) of the bio flavonoid extract in which double-wing insects larvae Chironomid Ch.riparius were put was 2280 mg/l. A semi-lethal dose (LD50) determined in laboratory mice at intra-abdominal administration was equal to 2375 mg/l. According to the toxicity classification of substances, the extract refers to the class of practically non- toxic substances. The herb golden pert extract has a weak ability of accumulation (TL50<12).

About the authors

Maria Nikolaevna Kurchatova

State Budgetary Educational Institution V.I. Razumovsky «Saratov State Medical University». RF Ministry of Health of Russia

Author for correspondence.
Email: noemail@neicon.ru

Аспирант кафедры общей биологии фармакогнозии и ботаники ГБОУ ВПО Саратовского государственного медицинского университета Минздрава РФ, 410012, г. Саратов, Российская Федерация,

e-mail: kurchatova.marya@yandex.ru

Russian Federation

Natalia Vladimirovna Polukonova

State Budgetary Educational Institution V.I. Razumovsky «Saratov State Medical University». RF Ministry of Health of Russia

Email: noemail@neicon.ru

Доктор биологических наук¸ профессор кафедры общей биологии фармакогнозии и ботаники ГБОУ ВПО Саратовского государственного Медицинского университета Минздрава РФ, 410012, г. Саратов, Российская Федерация,

e-mail: polukonovanv@yandex.ru 

Russian Federation

Natalia Anatolievna Durnova

State Budgetary Educational Institution V.I. Razumovsky «Saratov State Medical University». RF Ministry of Health of Russia

Email: noemail@neicon.ru

Доктор биологических наук, доцент кафедры общей биологии, фармакогнозии и ботаники ГБОУ ВПО Саратовского государственного медицинского университета Минздрава РФ, 410012, г. Саратов, Российская Федерация

e-mail: ndurnova@mail.ru

Russian Federation

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