In Vitro Evaluation of the Composite Alginate - Calcium Phosphate Materials for Prototyping Technologies in Bone Defects Substitution



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Abstract

Porous granular composite biomaterials based on sodium alginate and calcium phosphate component: 1% sodium alginate; sodium alginate with gelatin (80/20); calcium phosphate ceramics (CHA, TCP and OCP) and composite materials alginate/CHA, alginate/TCP, alginate/OCP, alginate/gelatin (80/20)/TCP and alginate/TCP/CHA/OCP were investigated for cytotoxicity and matrix (for cells) properties in dynamics of cultivation (up to 21 days). Two adhesive cell lines - immortalized human fibroblasts (HF, strain 1608 hTERT) and human osteosarcoma (MG-63) were used for in vitro MTT assay. OCP material showed its cytotoxicity while all its composites possessed weak matrix properties. For further research on the development of prototyping technologies 3 samples of composite materials were selected: alginate/CHA, alginate/TCP and alginate/gelatin (80/20)/TCP that are characterized by cytocompatibity and the presence of either expressed or moderate matrix properties of the surface for cells.

About the authors

N. S Sergeeva

P. A. Gertsen Moscow Research Oncology Institute

Email: prognoz.06@mail.ru

V. S Komlev

A. A. Baikov Institute of Metallurgy and Materials Science, Moscow, Russia

I. K Sviridova

P. A. Gertsen Moscow Research Oncology Institute

V. A Kirsanova

P. A. Gertsen Moscow Research Oncology Institute

S. A Akhmedova

P. A. Gertsen Moscow Research Oncology Institute

E. A Kuvshinova

P. A. Gertsen Moscow Research Oncology Institute

A. Yu Fedotov

A. A. Baikov Institute of Metallurgy and Materials Science, Moscow, Russia

A. Yu Teterina

A. A. Baikov Institute of Metallurgy and Materials Science, Moscow, Russia

S. M Barinov

A. A. Baikov Institute of Metallurgy and Materials Science, Moscow, Russia

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