Lncrna CASC15 Activated By TCF12 Promote Colorectal Cancer Progression via EMT


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Abstract

Background:Colorectal cancer (CRC) is one of the most common gastrointestinal malignancies. LncRNA CASC15 has also been found to play a vital role in malignant tumors.

Objective:Our objective is to explore the role of CASC15 in colorectal cancer and its regulation of EMT and to clarify the reasons for its up-regulated expression in CRC.

Methods:Quantitative real-time PCR was performed to evaluate the expression of CASC15 in CRC. The biology function of CASC15 on CRC was assessed by in vitro experiments, including CCK8, colony formation, transwell assays and flow cytometry. Luciferase reporter assays were used to confirm the regulation between TCF12 and CASC15. Quantitative real-time PCR and western blot analysis were used to evaluate the biomarkers associated with epithelial-mesenchymal transition (EMT).

Results:We found that CASC15 was remarkably upregulated in CRC and positively correlated with poorer relapse-free survival. CASC15 knockdown significantly suppressed the proliferation and migration of CRC. Furthermore, CASC15 downregulation mediated apoptosis of CRC. Mechanistically, TCF12 activates CASC15 transcription to mediate its up-regulation, which activates EMT and promotes CRC progression.

Conclusion:Our study identified TCF12/CASC15/EMT as a new regulatory signal axis of CRC. CASC15 may be a new molecular marker and target for CRC.

About the authors

Shuai Yan

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Ming Yuan

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Xichen Yang

Department of Pediatrics, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Xianting Huang

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Dan Wu

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Nanyao Wang

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Xia Sun

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Ya Qin

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Yanhua Fei

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Email: info@benthamscience.net

Zhenguo Zhao

Department of General Surgery, Jiangyin People's Hospital Affiliated to Nantong University

Author for correspondence.
Email: info@benthamscience.net

Qiong Wang

Department of Oncology, Jiangyin People's Hospital Affiliated to Nantong University

Author for correspondence.
Email: info@benthamscience.net

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