Аргентофильные ядрышковые организаторы и прогноз у больных поверхностным и местно-распространенным переходно-клеточным раком мочевого пузыря
- Авторы: Корнеев ИА1
-
Учреждения:
- Санкт-Петербургский государственный медицинский университет им. акад. И. П. Павлова
- Выпуск: № 1 (2005)
- Страницы: 5-9
- Раздел: Статьи
- URL: https://journals.eco-vector.com/1728-2985/article/view/276331
- ID: 276331
Цитировать
Полный текст
Аннотация
The aim of the study was to estimate distribution of the nucleoli and argentophilic nucleolar organizers in surface and locally advanced
transitional cell tumors of the urinary bladder (TCTUB), to analyse correlation between this distribution, tumor recurrence and survival of the patients. The data on primary examination and 5-year followup of 61 patients (47 male and 14 female, mean age 64 x 1 year) with surface and locally advanced TCTUB were studied retrospectively. AH the patients were radically operated on, 5-year progression-free survival made up 62%, recurrences of the urinary bladder tumors were detected in 61% patients. In addition to the standard morphological examination, tumor tissue was stained with silver nitrate according to the Howell and Black technique and the number of the nucleoli and argentophilic granules in them was counted in magnification lOOO.The findings were compared with the invasion depth, grade of cancer differentiation, tumor recurrence and 5-year survival. The count of argentophilic granules increased with reduction of differentiation grade and an increase of cancer invasion depth. Distribution of the nucleoli and granules significantly differed in the subgroups of patients with recurrent and recurrence-free course of the disease, depending on 5-year survival without progression. Probable duration of the recurrence-free period was estimated best by coefficient of variation of argentophilic granules distribution (p = 0.001). Parameters of distribution of the nucleoli and granules allowed specification of the risk of tumor progression for 5 years for all the patients (p = 0,035) and patients with surface tumors (p = 0.007) irrespective of categories T and G. Thus, the number of nucleoli and argentophilic nucleolar organizers increases with lowering of differentiation and deepening of tumor invasion. The analysis of nucleoli and argentophilic granules distribution provides additional information on recurrence and transient cell carcinomas probability.
transitional cell tumors of the urinary bladder (TCTUB), to analyse correlation between this distribution, tumor recurrence and survival of the patients. The data on primary examination and 5-year followup of 61 patients (47 male and 14 female, mean age 64 x 1 year) with surface and locally advanced TCTUB were studied retrospectively. AH the patients were radically operated on, 5-year progression-free survival made up 62%, recurrences of the urinary bladder tumors were detected in 61% patients. In addition to the standard morphological examination, tumor tissue was stained with silver nitrate according to the Howell and Black technique and the number of the nucleoli and argentophilic granules in them was counted in magnification lOOO.The findings were compared with the invasion depth, grade of cancer differentiation, tumor recurrence and 5-year survival. The count of argentophilic granules increased with reduction of differentiation grade and an increase of cancer invasion depth. Distribution of the nucleoli and granules significantly differed in the subgroups of patients with recurrent and recurrence-free course of the disease, depending on 5-year survival without progression. Probable duration of the recurrence-free period was estimated best by coefficient of variation of argentophilic granules distribution (p = 0.001). Parameters of distribution of the nucleoli and granules allowed specification of the risk of tumor progression for 5 years for all the patients (p = 0,035) and patients with surface tumors (p = 0.007) irrespective of categories T and G. Thus, the number of nucleoli and argentophilic nucleolar organizers increases with lowering of differentiation and deepening of tumor invasion. The analysis of nucleoli and argentophilic granules distribution provides additional information on recurrence and transient cell carcinomas probability.
Об авторах
И А Корнеев
Санкт-Петербургский государственный медицинский университет им. акад. И. П. ПавловаКафедра урологии; Санкт-Петербургский государственный медицинский университет им. акад. И. П. Павлова
Список литературы
- Матвеев Б. П. (ред.). Клиническая онкоурология. М.: Вердана; 2003.
- Кет W. Н. The grade and pathological stage of bladder cancer. Cancer (Philad.) 1984; 53: 1185-1189.
- Sobin L. H., Wittekind С. Urinary bladder. In: TNM classification of malignant tumours. 5th ed. New York: International Union Against Cancer, Willey-Liss; 1997. 187-190.
- Wolf H. Prognostic factors in bladder carcinoma. Scand. J. Urol. Nephrol. 1991; 138: 153-160.
- Аль-Шукри С. X., Ткачук В. Н. Опухоли мочеполовых органов. СПб.: Питер; 2000.
- Helpap В., Schmitz-Drager В. J., Hamilton P. W. et al. Molecular pathology of non-invasive urothelial carcinomas (part I). Virchows Arch. 2003; 442 (4): 309-316.
- Ooms E. С. М., Veldhuizen R. W. Argyrophilic proteins of the nucleolar organiser region in bladder tumors. Virchows Arch. Alt. A. Pathol. Anat. Histol. 1989; 414: 365-369.
- Мационис А. Э., Коган М. И., Матвеенко А. А. и др. Новые реальности цитологической диагностики рака мочевого пузыря. В кн.: Актуальные вопросы лечения онкоурологических заболеваний: Материалы 4-й Всероссийской науч. конф. с участием стран СНГ. М.: Российский онкологический науч. центр им. Н. Н. Блохина РАМН, Медицинский радиологический научный центр РАМН; 2001. 28-30.
- Calms P., Suarez V., Newman J., Crocker J. Nucleolar organizer regions in transitional cell tumors of the bladder. Arch. Pathol. Lab. Med. 1989; 113 (11): 1250-1252.
- Pick A., Chiusa L., Margaria E. Role of the argyrophilic nucleolar organizer regions in tumor detection and prognosis. Cancer Detect. Prev. 1995; 19 (3): 282-291.
- Lipponen P. K., Eskelinen M. J., Nordling S. Nucleolar organiser regions (AgNORs) as predictors in transitional cell bladder cancer. Br. J. Cancer 1991; 64: 1139-1144.
- Zambelli S., Zanin A., Gaglio A. et al. Ki-67 scores and AgNor counts in transitional cell carcinoma of the bladder: apparent lack of prognostic value. Arch. Ital. Urol. Androl. 1993; 65 (6): 665-670.
- Mostofi F. K., Sobin L. H., Torloni H. Histological typing of urinary bladder tumours. In: International histological classification of tumours. 10th ed. Geneva: World Health Organisation; 1973.
- Howell W. M., Black D. A. Controlled silver staining of nucleolus organiser regions with a protective colloidal developer: a one step method. Experientia 1980; 36: 1014-1015.
- Kurth K. H., Denis L., Bouffioux С. et al. Factors affecting recurrence and progression in superficial bladder tumors. Eur. J. Cancer 1995; 31A (11): 1840-1846.
- Morris S. В., Gordon E. M., Shearer R. J., Woodhouse С. R. J. Superficial bladder cancer: for how long should a tumour-free patient have check cystoscopies? Br. J. Urol. 1995; 75: 193-196.
Дополнительные файлы
